Response of the Hyperthermophilic ArchaeonSulfolobus solfataricusto UV Damage
Autor: | Christa Schleper, Mayi Arcellana Panlilio, Sabrina Fröls, Stephen D. Bell, Christoph Wilhelm Sensen, Iain G. Duggin, Paul M. K. Gordon |
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Rok vydání: | 2007 |
Předmět: |
DNA
Bacterial Time Factors Genomics and Proteomics DNA Repair Transcription Genetic Ultraviolet Rays Operon ved/biology.organism_classification_rank.species Biology Microbiology Direct DNA damage chemistry.chemical_compound Bacterial Proteins Extrachromosomal DNA Molecular Biology Gene Genetics ved/biology Cell Cycle Sulfolobus solfataricus Dose-Response Relationship Radiation Gene Expression Regulation Bacterial Cell biology chemistry Rad50 DNA microarray DNA DNA Damage |
Zdroj: | Journal of Bacteriology. 189:8708-8718 |
ISSN: | 1098-5530 0021-9193 |
Popis: | In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeoteSulfolobus solfataricusto UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements. While no significant up-regulation of genes potentially involved in direct DNA damage reversal was observed, a specific transcriptional UV response involving 55 genes could be dissected. Although flow cytometry showed only modest perturbation of the cell cycle, strong modulation of the transcript levels of the Cdc6 replication initiator genes was observed. Up-regulation of an operon encoding Mre11 and Rad50 homologs pointed to induction of recombinational repair. Consistent with this, DNA double-strand breaks were observed between 2 and 8 h after UV treatment, possibly resulting from replication fork collapse at damaged DNA sites. The strong transcriptional induction of genes which potentially encode functions for pilus formation suggested that conjugational activity might lead to enhanced exchange of genetic material. In support of this, a statistical microscopic analysis demonstrated that large cell aggregates formed upon UV exposure. Together, this provided supporting evidence to a link between recombinational repair and conjugation events. |
Databáze: | OpenAIRE |
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