Inhibitory effect of galanin on dopamine induced increased oxytocin secretion in rat neurohypophyseal tissue cultures
| Autor: | E. Csajbok, R. Toth, M. Galfi, Cs. Varga, Imre Pavo, F. A. László, Ferenc László, Éva Morschl, Lajos Baláspiri |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Physiology medicine.drug_class Dopamine Clinical Biochemistry Neuropeptide Galanin Biology Oxytocin Biochemistry Dopamine agonist Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Pituitary Gland Posterior Posterior pituitary Internal medicine medicine Animals Rats Wistar Neurotransmitter Dose-Response Relationship Drug Receptor antagonist Rats Apomorphine medicine.anatomical_structure chemistry medicine.drug |
| Zdroj: | Regulatory Peptides. 116:35-41 |
| ISSN: | 0167-0115 |
| DOI: | 10.1016/s0167-0115(03)00190-3 |
| Popis: | The regulation of oxytocin (OT) release by galanin (GAL) at the neurohypophyseal (NH) nerve terminal is not adequately understood. The effect of GAL on the secretion of OT was studied in 13- to 14-day cultures of isolated rat NH tissue. By this time, the hormone content of the medium had become constant. The OT content of the supernatant medium was determined by RIA after a 1- or 2-h incubation. A significantly decreased content of OT was found following incubation with 10(-6)-10(-8) M doses of GAL. Dopamine (DA) and the DA-active drugs apomorphine (APM) and Pro-Lys-Gly (PLG) (10(-6) M in each medium) increased the OT synthesis of NH tissue cultures. This elevation of OT secretion could be blocked by the administration of GAL together with DA, APM or PLG. The DA-blocking effect of GAL was prevented by previous treatment with the GAL receptor antagonist galantid (M15). The results indicate that OT release from the NH is directly influenced by the GAL-ergic system. The GAL-ergic control of OT secretion from NH tissue in rats can occur at the level of the posterior pituitary. |
| Databáze: | OpenAIRE |
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