Detection of Gold Nanoparticles Aggregation Growth Induced by Nucleic Acid through Laser Scanning Confocal Microscopy
| Autor: | G. Petriashvili, Giovani Carbone, Maria Penelope De Santo, Ramla Gary, Riccardo Barberi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
gold nanoparticle aggregation
deoxyribonucleic acid laser scanning confocal microscopy surface plasmon resonance Förster resonance energy transfer hydrophobicity Scanning electron microscope Confocal Metal Nanoparticles Nanotechnology 02 engineering and technology 010402 general chemistry lcsh:Chemical technology 01 natural sciences Biochemistry Article Analytical Chemistry Nucleic Acids Microscopy Fluorescence Resonance Energy Transfer lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Environmental scanning electron microscope Microscopy Confocal Chemistry Bilayer DNA 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Colloidal gold Biophysics Gold 0210 nano-technology |
| Zdroj: | Sensors, Vol 16, Iss 2, p 258 (2016) Sensors; Volume 16; Issue 2; Pages: 258 Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| Popis: | The gold nanoparticle (GNP) aggregation growth induced by deoxyribonucleic acid (DNA) is studied by laser scanning confocal and environmental scanning electron microscopies. As in the investigated case the direct light scattering analysis is not suitable, we observe the behavior of the fluorescence produced by a dye and we detect the aggregation by the shift and the broadening of the fluorescence peak. Results of laser scanning confocal microscopy images and the fluorescence emission spectra from lambda scan mode suggest, in fact, that the intruding of the hydrophobic moiety of the probe within the cationic surfactants bilayer film coating GNPs results in a Förster resonance energy transfer. The environmental scanning electron microscopy images show that DNA molecules act as template to assemble GNPs into three-dimensional structures which are reminiscent of the DNA helix. This study is useful to design better nanobiotechnological devices using GNPs and DNA. |
| Databáze: | OpenAIRE |
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