Altered levels of MALAT1 and H19 derived from serum or serum exosomes associated with type-2 diabetes
| Autor: | Eugenia Flores-Alfaro, Daniel Hernández-Sotelo, Mónica Ramírez, Marco Antonio Ramírez-Vargas, José Ángel Cahua-Pablo, Miguel Cruz, Oscar del-Moral-Hernández, Adakatia Armenta-Solís, Adan Valladares-Salgado, Vianet Argelia Tello-Flores |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Diabetes risk Waist lcsh:QH426-470 lncRNA-H19 lncRNA-MALAT1 Type 2 diabetes Exosomes Biochemistry Article 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal medicine Genetics Medicine Molecular Biology Type-2 diabetes MALAT1 business.industry Biochemistry (medical) medicine.disease Metabolic syndrome lcsh:Genetics 030104 developmental biology Endocrinology 030220 oncology & carcinogenesis Biomarker (medicine) Hemoglobin business |
| Zdroj: | Non-coding RNA Research, Vol 5, Iss 2, Pp 71-76 (2020) Non-coding RNA Research |
| ISSN: | 2468-0540 |
| DOI: | 10.1016/j.ncrna.2020.03.001 |
| Popis: | Environmental, genetic and epigenetic risk factors have been closely related to the development of type-2 diabetes (T2D). It has been reported that the expression in H19 and MALAT1 are related to metabolic diseases. To analyze the relationship between the expression of H19 and MALAT1 lncRNAs with diabetic patients. A study was conducted in subjects with T2D and nondiabetic controls, residents of Mexico City. Anthropometric measurements were made, and serum concentrations of glucose, glycosylated hemoglobin, total cholesterol, triglycerides, high- and low-density lipoprotein cholesterol were analyzed. Total RNA was extracted from serum and serum exosomes. The H19 and MALAT1 expression levels were quantified by RT-qPCR. A significant reduction in the expression of MALAT1 from serum or serum exosomes were found in patients with T2D, metabolic syndrome and low levels of HDL-c. Significant increase in H19 levels was found in diabetic subjects with poor glycemic control. Additionally, the principal component analyzes showed that serum MALAT1 expression was associated with total cholesterol and HDL-c levels, and the exosomes H19 expression was associated with waist circumference. The results obtained suggest that MALAT1 expression levels could be an epigenetic biomarker of diabetes risk or of its comorbidities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|