Early Pleistocene enamel proteome from Dmanisi resolves Stephanorhinus phylogeny
| Autor: | M. Thomas P. Gilbert, Ralf Dietrich Kahlke, Senthilvel K. S. S. Nathan, Yoshan Moodley, Bienvenido Martínez-Navarro, Lorenzo Rook, Luca Pandolfi, Jesper V. Olsen, Marc R. Dickinson, Reid Ferring, Jordi Agustí, Beth Shapiro, David Lyon, Christian D. Kelstrup, Jazmín Ramos-Madrigal, Morten E. Allentoft, Patrick Rüther, Mikkel-Holger S. Sinding, Anna K. Fotakis, David Lordkipanidze, Meaghan Mackie, Rosa Rakownikow Jersie-Christensen, Love Dalén, Eske Willerslev, Marcela Sandoval Velasco, Joshua D. Kapp, Gocha Kiladze, Aurélien Ginolhac, Frido Welker, Irina V. Kirillova, Peter D. Heintzman, J. Víctor Moreno-Mayar, Kirsty Penkman, Maia Bukhsianidze, Thomas W. Stafford, Ludovic Orlando, Diana Samodova, Enrico Cappellini, Martha Tappen, Shanlin Liu, Yvonne L. Chan, Anders Götherström, Eleftheria Palkopoulou |
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| Přispěvatelé: | University of Copenhagen = Københavns Universitet (KU), The National Museum of Georgia, LibraGen, industriel, Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden, Department of Zoology, University of Venda [South Africa], University of Venda, Institució Catalana de Recerca i Estudis Avançats (ICREA), Área de Prehistoria, URV-IPHES, Section for GeoGenetics, Globe Institute, Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU)-Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Georgian State Museum, Geology Paleontology and Paleoanthropology, Musée de Géorgie |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Male Proteomics History Early Pleistocene Proteome Lineage (evolution) [SDV]Life Sciences [q-bio] Amino Acid Motifs 01 natural sciences Woolly rhinoceros Naturvetenskap Phosphorylation ComputingMilieux_MISCELLANEOUS History Ancient Phylogeny Paleoproteomics Stephanorhinus Phylogeny Dmanisi Early Pleistocene Stephanorhinus Multidisciplinary Fossils Coelodonta DNA Ancient/analysis Sister group VDP::Mathematics and natural science: 400::Geosciences: 450::Stratigraphy and paleontology: 461 Natural Sciences Biotechnology General Science & Technology Dental Enamel/metabolism Perissodactyla/classification Biology 010603 evolutionary biology Article Ancient 03 medical and health sciences stomatognathic system Phylogenetics Genetics Proteome/analysis Animals Humans Amino Acid Sequence DNA Ancient Dental Enamel Perissodactyla VDP::Matematikk og Naturvitenskap: 400::Geofag: 450::Stratigrafi og paleontologi: 461 Bayes Theorem DNA 15. Life on land biology.organism_classification 030104 developmental biology Ancient DNA Evolutionary biology Phosphorylation/genetics |
| Zdroj: | Cappellini, E, Welker, F, Pandolfi, L, Ramos-Madrigal, J, Samodova, D, Rüther, P L, Fotakis, A K, Lyon, D, Moreno-Mayar, J V, Bukhsianidze, M, Rakownikow Jersie-Christensen, R, Mackie, M, Ginolhac, A, Ferring, R, Tappen, M, Palkopoulou, E, Dickinson, M R, Stafford, T W, Chan, Y L, Götherström, A, Nathan, S K S S, Heintzman, P D, Kapp, J D, Kirillova, I, Moodley, Y, Agusti, J, Kahlke, R D, Kiladze, G, Martínez-Navarro, B, Liu, S, Sandoval Velasco, M, Sinding, M H S, Kelstrup, C D, Allentoft, M E, Orlando, L, Penkman, K, Shapiro, B, Rook, L, Dalén, L, Gilbert, M T P, Olsen, J V, Lordkipanidze, D & Willerslev, E 2019, ' Early Pleistocene enamel proteome from Dmanisi resolves Stephanorhinus phylogeny ', Nature, vol. 574, no. 7776, pp. 103-107 . https://doi.org/10.1038/s41586-019-1555-y Nature, vol 574, iss 7776 Nature Nature, Nature Publishing Group, 2019, 574 (7776), pp.103-107. ⟨10.1038/s41586-019-1555-y⟩ |
| ISSN: | 0028-0836 1476-4679 |
| DOI: | 10.1038/s41586-019-1555-y |
| Popis: | The sequencing of ancient DNA has enabled the reconstruction of speciation, migration and admixture events for extinct taxa1. However, the irreversible post-mortem degradation2 of ancient DNA has so far limited its recovery—outside permafrost areas—to specimens that are not older than approximately 0.5 million years (Myr)3. By contrast, tandem mass spectrometry has enabled the sequencing of approximately 1.5-Myr-old collagen type I4, and suggested the presence of protein residues in fossils of the Cretaceous period5—although with limited phylogenetic use6. In the absence of molecular evidence, the speciation of several extinct species of the Early and Middle Pleistocene epoch remains contentious. Here we address the phylogenetic relationships of the Eurasian Rhinocerotidae of the Pleistocene epoch7–9, using the proteome of dental enamel from a Stephanorhinus tooth that is approximately 1.77-Myr old, recovered from the archaeological site of Dmanisi (South Caucasus, Georgia)10. Molecular phylogenetic analyses place this Stephanorhinus as a sister group to the clade formed by the woolly rhinoceros (Coelodonta antiquitatis) and Merck’s rhinoceros (Stephanorhinus kirchbergensis). We show that Coelodonta evolved from an early Stephanorhinus lineage, and that this latter genus includes at least two distinct evolutionary lines. The genus Stephanorhinus is therefore currently paraphyletic, and its systematic revision is needed. We demonstrate that sequencing the proteome of Early Pleistocene dental enamel overcomes the limitations of phylogenetic inference based on ancient collagen or DNA. Our approach also provides additional information about the sex and taxonomic assignment of other specimens from Dmanisi. Our findings reveal that proteomic investigation of ancient dental enamel—which is the hardest tissue in vertebrates11, and is highly abundant in the fossil record—can push the reconstruction of molecular evolution further back into the Early Pleistocene epoch, beyond the currently known limits of ancient DNA preservation. Palaeoproteomic analysis of dental enamel from an Early Pleistocene Stephanorhinus resolves the phylogeny of Eurasian Rhinocerotidae, by enabling the reconstruction of molecular evolution beyond the limits of ancient DNA preservation. |
| Databáze: | OpenAIRE |
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