Comparative gene expression profiles in pancreatic islets associated with agouti yellow mutation and PACAP overexpression in mice
| Autor: | Shuhei Tomimoto, Hitoshi Hashimoto, Kazuya Ikeda, Takanobu Nakazawa, Kazuki Nagayasu, Atsuko Hayata-Takano, Atsushi Kasai, Soken Tsuchiya, Yukihiko Sugimoto, Ken Ichi Hamagami, Norihito Shintani, Atsushi Ichikawa, Akemichi Baba |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetically modified mouse
medicine.medical_specialty endocrine system Pancreatic islet hyperplasia PACAP pituitary adenylate cyclase-activating polypeptide Biophysics Biology medicine.disease_cause PACAP Lethal yellow agouti (KKAy) diabetic mice Chromosome Biochemistry Internal medicine Gene expression KKAy mice lethal yellow agouti diabetic mice medicine Hyperinsulinemia Mitochondrion Gene GO gene ontology geography Mutation geography.geographical_feature_category Pancreatic islets PACAP-Tg mice transgenic mice overexpressing PACAP in pancreatic β-cells Islet medicine.disease Ribosome Endocrinology medicine.anatomical_structure hormones hormone substitutes and hormone antagonists Research Article |
| Zdroj: | Biochemistry and Biophysics Reports |
| ISSN: | 2405-5808 |
| Popis: | In diabetes mellitus, pituitary adenylate cyclase-activating polypeptide (PACAP) has insulinotropic and glucose-lowering properties. We previously demonstrated that transgenic mice overexpressing PACAP in pancreatic β-cells (PACAP-Tg) show attenuated pancreatic islet hyperplasia and hyperinsulinemia in type 2 diabetic models. To explore the underlying mechanisms, here we crossed PACAP-Tg mice with lethal yellow agouti (KKAy) diabetic mice, and performed gene chip analysis of laser capture microdissected pancreatic islets from four F1 offspring genotypes (wild-type, PACAP-Tg, KKAy, and PACAP-Tg:KKAy). We identified 1371 probes with >16-fold differences between at least one pair of genotypes, and classified the probes into five clusters with characteristic expression patterns. Gene ontology enrichment analysis showed that genes involved in the terms ribosome and intracellular organelles such as ribonucleoprotein complex, mitochondrion, and chromosome organization were significantly enriched in clusters characterized by up-regulated genes in PACAP-Tg:KKAy mice compared with KKAy mice. These results may provide insight into the mechanisms of diabetes that accompany islet hyperplasia and amelioration by PACAP. Highlights • PACAP overexpressed in KKAy diabetic mice is known to exert antidiabetic effects. • We performed gene chip analysis of pancreatic islets in these mice. • Gene ontology analysis was performed for genes classified into five clusters. • Genes involved in the terms ribosome, mitochondrion, and chromosome were enriched. • These pathways may be involved in the mechanism by which PACAP ameliorates diabetes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|