Two-dimensional planar swimming selects for high DNA integrity sperm
| Autor: | David Sinton, Yihe Wang, Biao Zhang, Farhang Tarlan, Thomas Hannam, Keith Jarvi, Percival J. Graham, Jae Bem You, Alexander Lagunov, Jason Riordon, Tian Kong |
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| Rok vydání: | 2019 |
| Předmět: |
Male
endocrine system Sperm Head Biomedical Engineering Bioengineering 02 engineering and technology Reproductive technology Biology 01 natural sciences Biochemistry Centrifugation Density Gradient Semen sample Humans reproductive and urinary physiology Sperm motility Selection (genetic algorithm) Dna integrity urogenital system 010401 analytical chemistry DNA General Chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Spermatozoa Sperm 0104 chemical sciences Cell biology Sperm Motility Selection method 0210 nano-technology human activities |
| Zdroj: | Lab on a Chip. 19:2161-2167 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | Selection of high-quality sperm is critical to the success of assisted reproductive technologies. Clinical screening for top sperm has long focused on sperm swimming ability when following boundaries or when fully free of constraints. In this work, we demonstrate a sperm selection approach with parallel 2 μm tall confined selection channels that prohibit rotation of the sperm head and require planar swimming. We demonstrate that a planar swimming subpopulation of sperm capable of entering and navigating these channels has DNA integrity superior to the freely-swimming motile or raw sperm populations over a wide range of semen sample qualities. The DNA integrity of the selected sperm was significantly higher than that of the corresponding raw samples for donor samples and clinical patient samples, respectively. In side-by-side testing, this method outperforms current clinical selection methods, density gradient centrifugation and swim-up, as well as sperm selected via general motility. Planar swimming represents a viable sperm selection mechanism with the potential to improve outcomes for couples and offspring. |
| Databáze: | OpenAIRE |
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