An In Vitro Method for Screening Compounds for the Effect on the Rate of Sickling of Erythrocytes
| Autor: | R D Mackenzie, G L Schatzman, E M Gleason, M J Cawein |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Erythrocytes
Glutamine Anemia Sickle Cell 030204 cardiovascular system & hematology Pharmacology Biochemistry 03 medical and health sciences Procaine 0302 clinical medicine Antisickling Agents In vivo Calcium flux Methods medicine Humans Inhibitory effect Whole blood 2-Hydroxyphenethylamine Aniline Compounds business.industry Erythrocyte Membrane Biochemistry (medical) Oxygen–haemoglobin dissociation curve Cell Biology General Medicine In vitro Oxygen Ethanolamines 030220 oncology & carcinogenesis Calcium concentration Calcium business medicine.drug |
| Zdroj: | Journal of International Medical Research. 4:375-381 |
| ISSN: | 1473-2300 0300-0605 |
| DOI: | 10.1177/030006057600400602 |
| Popis: | Since no practical animal model is available for the evaluation of compounds in vivo, we have developed an in vitro model for determining the effect of compounds on the rate of sickling of erythrocytes in whole blood taken from patients with sickle cell anaemia. RMI 6792 (a phenethanol-diamine derivative), procaine, and L-glutamine were tested in this in vitro system. RMI 6792 was tested at various concentrations in whole blood. The data indicate that RMI 6792 decreased the rate of sickling at and above 60 μg/ml. Procaine slightly decreased sickling rate at 100 μg/ml. L-glutamine at 555 μg/ml had no inhibitory effect. RMI 6792 and procaine had no effect on the oxygen dissociation curve. RMI 6792 affected the calcium flux of the erythrocytes and the calcium concentration in the erythrocytes. |
| Databáze: | OpenAIRE |
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