Multiple Mechanisms Contribute to Lateral Transfer of an Organophosphate Degradation (opd) Island in Sphingobium fuliginis ATCC 27551
| Autor: | Anil K. Madugundu, Sunil Parthasarathy, Dayananda Siddavattam, Emmanuel Vijay Paul Pandeeti, Sujana Ghanta, Srikanth Reddy Medipally, Venkateswar Reddy Muthyala, Surat Chameli Pantula, Deviprasanna Chakka, Toshisangba Longkumer, Harshita Yekkala |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Transposable element
Serine-tRNA Ligase Gene Transfer Horizontal catabolic transposons Molecular Sequence Data Addiction module Replication Origin Biology Investigations genomic islands phosphotriesterase (PTE) Plasmid Bacterial Proteins Host chromosome Gene cluster Genetics Escherichia coli Replicon Cloning Molecular Molecular Biology Gene Genetics (clinical) Recombination Genetic Base Sequence Integrases integrative conjugative elements (ICE) Molecular biology Organophosphates Sphingomonadaceae Genes Bacterial Multigene Family Attachment Sites Microbiological Sphingobium japonicum Plasmids |
| Zdroj: | G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | The complete sequence of pPDL2 (37,317 bp), an indigenous plasmid of Sphingobium fuliginis ATCC 27551 that encodes genes for organophosphate degradation (opd), revealed the existence of a site-specific integrase (int) gene with an attachment site attP, typically seen in integrative mobilizable elements (IME). In agreement with this sequence information, site-specific recombination was observed between pPDL2 and an artificial plasmid having a temperature-sensitive replicon and a cloned attB site at the 3′ end of the seryl tRNA gene of Sphingobium japonicum. The opd gene cluster on pPDL2 was found to be part of an active catabolic transposon with mobile elements y4qE and Tn3 at its flanking ends. Besides the previously reported opd cluster, this transposon contains genes coding for protocatechuate dioxygenase and for two transport proteins from the major facilitator family that are predicted to be involved in transport and metabolism of aromatic compounds. A pPDL2 derivative, pPDL2-K, was horizontally transferred into Escherichia coli and Acinetobacter strains, suggesting that the oriT identified in pPDL2 is functional. A well-defined replicative origin (oriV), repA was identified along with a plasmid addiction module relB/relE that would support stable maintenance of pPDL2 in Sphingobium fuliginis ATCC 27551. However, if pPDL2 is laterally transferred into hosts that do not support its replication, the opd cluster appears to integrate into the host chromosome, either through transposition or through site-specific integration. The data presented in this study help to explain the existence of identical opd genes among soil bacteria. |
| Databáze: | OpenAIRE |
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