Carbonic anhydrase from Apis mellifera: purification and inhibition by pesticides
| Autor: | Deniz Ekinci, Ercan Soydan, Ahmet Güler, Claudiu T. Supuran, Murat Şentürk, Selim Biyik |
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| Přispěvatelé: | Belirlenecek, OMÜ |
| Rok vydání: | 2017 |
| Předmět: |
carbonic anhydrase
Simazine 01 natural sciences Chromatography Affinity chemistry.chemical_compound Carbonic anhydrase Carbaryl Drug Discovery Animals Atrazine Pesticides Carbonic Anhydrase Inhibitors pesticide Carbonic Anhydrases Pharmacology chemistry.chemical_classification Chromatography biology 010405 organic chemistry lcsh:RM1-950 General Medicine Propoxur Pesticide Bees 0104 chemical sciences inhibitor 010404 medicinal & biomolecular chemistry lcsh:Therapeutics. Pharmacology Enzyme chemistry Biochemistry biology.protein Electrophoresis Polyacrylamide Gel Apis mellifera Carbofuran Research Article |
| Zdroj: | Journal of Enzyme Inhibition and Medicinal Chemistry Journal of Enzyme Inhibition and Medicinal Chemistry, Vol 32, Iss 1, Pp 47-50 (2017) |
| ISSN: | 1475-6374 |
| Popis: | BIYIK, Selim/0000-0003-4152-2596; GULER, Ahmet/0000-0003-0167-2346 WOS: 000392591100038 PubMed: 28090787 Carbonic anhydrase (CA) enzymes have been shown to play an important role in ion transport and in pH regulation in several organisms. Despite this information and the wealth of knowledge regarding the significance of CA enzymes, few studies have been reported about bee CA enzymes and the hazardous effects of chemicals. Using Apis mellifera as a model, this study aimed to determine the risk of pesticides on Apis mellifera Carbonic anhydrase enzyme (Am CA). CA was initially purified from Apis mellifera spermatheca for the first time in the literature. The enzyme was purified with an overall purification of similar to 35-fold with a molecular weight of similar to 32 kDa. The enzyme was then exposed to pesticides, including tebuconazole, propoxur, carbaryl, carbofuran, simazine and atrazine. The six pesticides dose-dependently inhibited in vitro AmCA activity at low micromolar concentrations. IC50 values for the pesticides were 0.0030, 0.0321, 0.0031, 0.0087, 0.0273 and 0.0165 mu M, respectively. The AmCA inhibition mechanism of these compounds is unknown at this moment. |
| Databáze: | OpenAIRE |
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