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Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein production, viral vaccines and gene therapy application. The transfection work was initially limited to small-scale operations. Operation of suspension cultures and the removal of serum from the medium for transfection were major improvements that made it possible to advance this technology to large-scale operations. PEI-DNA condensation method has been preferred among the researchers to further the advancement of transfection technology. Transient cell transfection technology is a powerful tool to deliver sufficient quantities of recombinant human therapeutic proteins in a cost-effective manner within a couple of weeks. In addition, progress in large-scale transfection technology has been successfully implemented in efficient manufacturing of viral vectors for gene therapy. |
