Trisomy 3 in marginal zone B-cell lymphoma: a study based on cytogenetic analysis and fluorescence in situ hybridization
| Autor: | J J Cassiman, J. Thomas, Pia Delaere, H. Van den Berghe, I. Wlodarska, Michel Stul, Arnold Criel, Christina Mecucci, C De Wolf-Peeters, Marc Boogaerts, W. Zeller, Judith Dierlamm, Stefania Pittaluga, Lucienne Michaux |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Lymphoma B-Cell Aneuploidy Trisomy Chromosomal translocation Biology medicine Humans Interphase In Situ Hybridization Fluorescence Metaphase Aged Aged 80 and over medicine.diagnostic_test Cytogenetics Karyotype Hematology Middle Aged medicine.disease Chromosome 3 Karyotyping Female Marginal zone B-cell lymphoma Chromosomes Human Pair 3 Fluorescence in situ hybridization |
| Zdroj: | British Journal of Haematology. 93:242-249 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1046/j.1365-2141.1996.522522.x |
| Popis: | Trisomy 3 represents the most frequent and consistent chromosomal abnormality characterizing the recently defined entity marginal zone B-cell lymphoma (MZBCL). By cytogenetic analysis and/or fluorescence in situ hybridization (FISH) on interphase nuclei we found an increased copy number of chromosome 3 in 22/36 (61%) successfully analysed cases, including 8/12 cases with extranodal MZBCL, 8/13 cases with nodal MZBCL, and 6/11 patients with splenic MZBCL. Sensitivity of interphase cytogenetics was somewhat higher than that of conventional cytogenetic investigation, Structural chromosomal changes involving at least one chromosome 3 were seen in 11/20 cases with an increased copy number of chromosome 3: +del(3)(p13) was demonstrated in three cases, and was the sole chromosomal abnormality in one of them: +i(3)(q10) was seen in two other patients; and rearrangements involving various breakpoints on the long arm of chromosome 3 were found in the remaining cases, FISH on metaphase spreads confirmed these structural abnormalities and additionally showed two unexpected translocations involving chromosome 3, We conclude that: (1) trisomy 3 occurs in a high proportion of extranodal, nodal and splenic MZBCL; (2) FISH on interphase nuclei is an additional and sensitive tool in detecting an increased copy number of chromosome 3 in MZBCL; (3) additional structural abnormalities involving the long arm of chromosome 3 are frequent but non-recurrent and are perhaps secondary changes; and (4) abnormalities such as +del(3)(p13) and +i(3)(q10) suggest that genes located on the long arm of chromosome 3 are of particular importance in the pathogenesis of MZBCL. |
| Databáze: | OpenAIRE |
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