Identification of disordered metabolic networks in postpartum dairy cows with left displacement of the abomasum through integrated metabolomics and pathway analyses
| Autor: | Sheng Hu He, Fan Hua Wei, Yan Sheng Guo, Li Hua Xu, Jin Zhong Tao |
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| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
left displacement of the abomasum Cattle Diseases Phenylalanine postpartum dairy cow Mass Spectrometry 0403 veterinary science 03 medical and health sciences Metabolomics Valine Internal Medicine Animals 030304 developmental biology 0303 health sciences Full Paper General Veterinary Chemistry Abomasum Postpartum Period 04 agricultural and veterinary sciences Metabolism metabolomics pathway analysis Metabolic pathway Biochemistry Pyrimidine metabolism Metabolome Ketone bodies Cattle Female Leucine liquid chromatography coupled with quadrupole time of flight mass spectrometry Biomarkers Metabolic Networks and Pathways Chromatography Liquid |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.19-0378 |
| Popis: | High-producing dairy cows are easily affected by left displacement of the abomasum (LDA) within 4 weeks postpartum. Although LDA is highly associated with metabolic disturbances, the related information on comprehensive metabolic changes, with the exception of some blood biochemical parameters, remains limited. In this study, the changes in plasma metabolites and in the metabolic profile of postpartum dairy cows with LDA were investigated through liquid chromatography coupled with quadrupole time of flight mass spectrometry (LC-Q/TOF-MS)-based metabolomics, and the metabolic networks related to LDA were constructed through metabolomics pathway analysis (MetPA). An obvious change in the metabolic profile was reflected by significant variations in 68 plasma metabolites in postpartum dairy cows with LDA, and these variations consequently altered 13 metabolic pathways (histidine metabolism, tyrosine metabolism, valine, leucine and isoleucine biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, arginine and proline metabolism, tryptophan metabolism, synthesis and degradation of ketone bodies, linoleic acid metabolism, arachidonic acid metabolism, citrate cycle, butanoate metabolism, vitamin B6 metabolism and pyrimidine metabolism). This study shows that the more detailed information obtained by LC-Q/TOF-MS-based metabolomics and MetPA might contribute to a better understanding of the disordered metabolic networks in postpartum dairy cows with LDA. |
| Databáze: | OpenAIRE |
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