Molecular characterization of a glycerol-3-phosphate acyltransferase reveals key features essential for triacylglycerol production in Phaeodactylum tricornutum
| Autor: | Da-Wei Li, Jie-Sheng Liu, Srinivasan Balamurugan, Dong‑Xiong Hu, Hong-Ye Li, Ying Fang Niu, Wei-Dong Yang, Xiang Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Management Monitoring Policy and Law Applied Microbiology and Biotechnology Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Biosynthesis GPAT Phaeodactylum tricornutum Diatoms chemistry.chemical_classification biology Renewable Energy Sustainability and the Environment Research Nile red Lipid metabolism Metabolism Lipid biology.organism_classification 030104 developmental biology General Energy Enzyme Biochemistry chemistry Biofuels Acyltransferase Biotechnology |
| Zdroj: | Biotechnology for Biofuels |
| ISSN: | 1754-6834 |
| DOI: | 10.1186/s13068-016-0478-1 |
| Popis: | Background The marine diatom, Phaeodactylum tricornutum, has become a model for studying lipid metabolism and its triacylglycerol (TAG) synthesis pathway makes it an ideal target for metabolic engineering to improve lipid productivity. However, the genetic background and metabolic networks of fatty acid biosynthesis in diatoms are not well understood. Glycerol-3-phosphate acyltransferase (GPAT) is the critical enzyme that catalyzes the first step of TAG formation. So far, characterization of GPAT in marine microalgae has not been reported, especially at the level of comprehensive sequence-structure and functional analysis. Results A GPAT was cloned from P. tricornutum and overexpressed in P. tricornutum. Volumes of oil bodies were produced and the neutral lipid content was increased by twofold determined by Nile red fluorescence staining. Fatty acid composition was analyzed by GC–MS, which showed significantly higher proportion of unsaturated fatty acids compared to wild type. Conclusion These results suggested that the identified GPAT could upregulate TAG biosynthesis in P. tricornutum. Moreover, this study offers insight into the lipid metabolism of diatoms and supports the role of microalgal strains for biofuels production. |
| Databáze: | OpenAIRE |
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