A Sensitive Immunoblotting Technique for the Serodiagnosis of Brucella Ovis Infections
| Autor: | Mike F. Hansen, R Kittelberger, F Hilbink, G.P. Ross |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Brucella ovis 040301 veterinary sciences Immunoblotting Sheep Diseases Enzyme-Linked Immunosorbent Assay Semen Biology Sensitivity and Specificity Brucellosis Microbiology 0403 veterinary science chemistry.chemical_compound Antigen Animals Sodium dodecyl sulfate Ovis Sheep General Veterinary Complement Fixation Tests 0402 animal and dairy science 04 agricultural and veterinary sciences biology.organism_classification Complement fixation test Brucella 040201 dairy & animal science chemistry biology.protein Electrophoresis Polyacrylamide Gel Female Flock Antibody |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 6:188-194 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | A simplified electrophoretic immunoblotting technique based on antigen extracted from Brucella ovis cells with sodium dodecyl sulfate/mercaptoethanol was compared with the complement fixation test (CFT), the enzyme-linked immunosorbent assay, and the gel diffusion test. Sera from 89 chronically infected, semen culture-positive rams, 378 sera from B. ovis-infected flocks, 300 sera from accredited disease-free flocks, and 29 sera from specific-pathogen-free sheep were used. The immunoblotting technique had sensitivity and specificity comparable to those of the standard tests and was able to identify several CFT-negative or -borderline sera as positive. The major immunoreactive antigens of B. ovis had molecular masses of 63,29, 19 kD (proteins) and 8-12 kD (rough lipopolysaccharide). Antibodies against these antigens were present in 96% of CFT-positive sera from infected flocks and in 100% of sera from semen culture-positive rams. However, immunoblotting also identified antibodies to components other than the major antigens in 1% of CFT-negative sera from infected flocks and in 7.7% of the sera from flocks with a history of freedom from the disease. These reactions probably represent cross-reactivities with other microorganisms and were distinguishable from truly positive reactions. |
| Databáze: | OpenAIRE |
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