Characterization of β-adrenergic receptors of freshly isolated astrocytes and neurons from rat brain
| Autor: | Roland Zini, Rosa Sapena, Didier Morin, Jean-Paul Tillement, Brigitte Onteniente |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Cell type Cerebellum High affinity binding Adrenergic beta-Antagonists Biology Binding Competitive General Biochemistry Genetics and Molecular Biology Propanolamines Prosencephalon Receptors Adrenergic beta medicine Animals Rats Wistar General Pharmacology Toxicology and Pharmaceutics Receptor Cells Cultured Neurons Imidazoles General Medicine Adrenergic beta-Agonists Rat brain Immunohistochemistry Rats medicine.anatomical_structure Cerebral cortex Astrocytes Forebrain Biophysics β adrenergic receptor Neuroscience |
| Zdroj: | Life Sciences. 60:315-324 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(96)00632-7 |
| Popis: | The binding and characteristics of rat brain beta-adrenergic receptors (beta-AR) isolated from astrocytes and neurons were investigated. Equilibrium binding experiments demonstrated that beta-AR were more concentrated on astrocytes than on neurons isolated from forebrain, cerebral cortex and cerebellum. Inhibition experiments revealed that beta 1-AR and beta 2-AR were present in the two cell types. Isoproterenol revealed two interchangeable states of high and low affinity binding to both beta 1- and beta 2-AR in neurons. The high affinity binding sites were sensitive to guanylylimidodiphosphate (GppNHp). Similar results were found with other beta-AR agonists but not with salbutamol and salmeterol which recognized both affinity states of the neuronal beta 2-AR but only the low affinity state of beta 1-AR. In astrocytes only the low affinity state of beta-AR was observed. |
| Databáze: | OpenAIRE |
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