Three novel rhamnogalacturonan I- pectins degrading enzymes from Aspergillus aculeatinus: Biochemical characterization and application potential
| Autor: | Adrien Lemaire, Jérôme Pelloux, Valérie Lefebvre, Corinne Pau-Roblot, Solène Bassard, Catalina Duran Garzon, Olivier Habrylo, Aurore Perrin, Pauline Trezel, Anaïs Guillaume, Olivier Van Wuytswinkel |
|---|---|
| Přispěvatelé: | Transfrontalière BioEcoAgro (Transfrontalière BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA) |
| Rok vydání: | 2020 |
| Předmět: |
Magnetic Resonance Spectroscopy
Glycoside Hydrolases Polymers and Plastics 02 engineering and technology 010402 general chemistry 01 natural sciences Pichia Pichia pastoris Fungal Proteins Cell wall Cell Wall Enzyme Stability Materials Chemistry [CHIM]Chemical Sciences Mode of action ComputingMilieux_MISCELLANEOUS Polysaccharide-Lyases chemistry.chemical_classification biology fungi Organic Chemistry Temperature food and beverages Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification Recombinant Proteins Camelina 0104 chemical sciences Kinetics Aspergillus Enzyme Mucilage chemistry Biochemistry Pectins Degradation (geology) 0210 nano-technology Digestion |
| Zdroj: | Carbohydrate Polymers Carbohydrate Polymers, Elsevier, 2020, 248, pp.116752-. ⟨10.1016/j.carbpol.2020.116752⟩ Carbohydrate Polymers, 2020, 248, pp.116752-. ⟨10.1016/j.carbpol.2020.116752⟩ |
| ISSN: | 0144-8617 |
| Popis: | Rhamnogalaturonans I (RGI) pectins, which are a major component of the plant primary cell wall, can be recalcitrant to digestion by commercial enzymatic cocktails, in particular during fruit juice clarification process. To overcome these problems and get better insights into RGI degradation, three RGI degrading enzymes (RHG: Endo-rhamnogalacturonase; ABF: α-Arabinofuranosidases; GAN: Endo-β-1,4-galactanase) from Aspergillus aculeatinus were expressed in Pichia pastoris, purified and fully biochemically characterized. All three enzymes showed acidic pH optimum, and temperature optima between 40-50 °C. The Km values were 0.5 mg.ml-1, 1.64 mg.ml-1 and 3.72 mg.ml-1 for RHG, ABF, GAN, respectively. NMR analysis confirmed an endo-acting mode of action for RHG and GAN, and exo-acting mode for ABF. The application potential of these enzymes was assessed by measuring changes in viscosity of RGI-rich camelina mucilage, showing that RHG-GAN enzymes induced a decrease in viscosity by altering the structures of the RGI backbone and sidechains. |
| Databáze: | OpenAIRE |
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