Murine fascioliasis: laboratory model for the detection of fasciolicidal activity of chemical compounds
| Autor: | Hayes Tj, Mitrovic M, Bailer J |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Male
Fascioliasis Hexachlorophene Catechols Oxyclozanide Pharmacology Sulfides Nitrophenols chemistry.chemical_compound Mice Phenols Hepatica Nitriles Salicylamides medicine Fasciola hepatica Animals Anilides Pathogen Ecology Evolution Behavior and Systematics Anthelmintics biology Diamphenethide biology.organism_classification Bromine Rafoxanide Disease Models Animal Bithionol chemistry Parasitology Chlorine medicine.drug Iodine |
| Zdroj: | The Journal of parasitology. 59(2) |
| ISSN: | 0022-3395 |
| Popis: | Rafoxanide, oxyclozanide, nitroxynil, hilomid, bithionol, hexachlorophene, and clioxanide were all active against immature F. hepatica in mice when given in the diet from 14 to 21 days postinfection. In infected unmedicated mice, hemoperitoneum, hepatic necrosis, capsulitis, and recent lesions occurred in 90% or more of the infected animals at 28 days postinfection with 2 metacercariae. The activities of established fasciolicidal drugs could be clearly shown by a reduction in pathognostic pathology in infected medicated mice at 28 days postinfection. Based on the results, a method suitable for the primary screening of new chemical compounds is given. Primary empirical screening of new chemical compounds for activity against any particular pathogen is most readily accomplished in a laboratory model of the disease in question. In the case of fascioliasis, the most widely employed system has been experimental infection of the rat with Fasciola hepatica (Lammler, 1968; Boray, 1969). Although recognized as an excellent laboratory host of F. hepatica, the mouse (Dawes and Hughes, 1964, 1970) has received little attention as a possible host for screening purposes. Happich (unpublished work cited in Boray, loc. cit.) tested four known active drugs at two dose levels in mice and found that the drugs were either toxic or inactive, and thus concluded that the mouse may not be a suitable host in which to screen potential compounds for fasciolicidal activity. Nevertheless, at least two of the newer fasciolicidal drugs, oxyclozanide (Broome and Jones, 1966) and diamphenethide (Dickerson et al., 1971), seem to have been initially detected in a mouse system. A recent report by Kruyt and van der Steen (1971) suggests that a F. hepatica-mouse test model may be useful for screening purposes. At present, however, no published screening methods in mice are available. This report describes the effects of several established fasciolicidal drugs given in the diet from 14 to 21 days postinfection (PI) on F. hepatica in mice. Emphasis is placed on alteration of pathologic status, and a method for the primary screening of chemicals for activity against F. hepatica is given, based on the results. Received for publication 24 November 1972. MATERIALS AND METHODS |
| Databáze: | OpenAIRE |
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