A Mouse and Embryonic Stem Cell Derived from a Single Embryo
| Autor: | Toshiyuki Takehara, Naoko Kishi, Toshihiro Mihara, Nobuyuki Kawata, Kazuhiro Saeki, Kazuya Matsumoto, Makoto Takenoshita, Norimasa Sagawa, Yoshihiko Hosoi, Hiroki Takeuchi, Akira Iritani, Takeshi Teramura |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Genotype Biopsy Cloning Organism Biology Polymerase Chain Reaction Mice Chimera (genetics) Animals Embryonic Stem Cells reproductive and urinary physiology Genetics Chimera urogenital system Drug discovery Teratoma Embryo Karyotype Blastomere Embryo Transfer Immunohistochemistry Embryonic stem cell Genetically modified organism Cell biology embryonic structures Female biological phenomena cell phenomena and immunity Microsatellite Repeats Developmental Biology Biotechnology |
| Zdroj: | Cloning and Stem Cells. 9:485-494 |
| ISSN: | 1557-7457 1536-2302 |
| Popis: | Embryonic stem cells (ESCs) are a good material for the study of mammalian development, production of genetically modified animals, and drug discovery because they proliferate infinitely while maintaining a multilineage differentiation potency and a normal karyotype. However, ethical considerations limit the use of human embryos for the establishment of ESCs. Recently, ESCs have been produced from blastomeres divided by biopsy in mice and humans. The method is expected to be less controversial because it does not destroy the embryo. However, no one has yet produced both a pup and an ESC from a single embryo. Here, we describe the production of individual/ESC pairs from each of three embryos out of 20 attempts, and is thus considered efficient. Blastomere-derived ESC could differentiate some types of tissues and contribute to chimera mouse. These results show that each blastomere at two-cell stage possesses pluripotency and separated blastomeres maintain viability to develop to a pup or pluripotent ESC. |
| Databáze: | OpenAIRE |
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