Quantum Dot Lipid Oligonucleotide Bioconjugates: Toward a New Anti-MicroRNA Nanoplatform
| Autor: | Isabelle Bestel, Ahissan Aimé, Nataliia Beztsinna, Amit Patwa, Alexander Pokolenko, Philippe Barthélémy |
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| Rok vydání: | 2013 |
| Předmět: |
Intracellular Space
Oligonucleotides Biomedical Engineering Pharmaceutical Science Bioengineering Nanotechnology Quantum Dots microRNA Humans Semiconductor nanocrystals Pharmacology Base Sequence Chemistry Oligonucleotide Organic Chemistry technology industry and agriculture Nucleic Acid Hybridization Biological Transport equipment and supplies Lipids Fluorescence Kinetics MicroRNAs Semiconductors Solubility Quantum dot bacteria Hydrophobic and Hydrophilic Interactions HeLa Cells Biotechnology Conjugate |
| Zdroj: | Bioconjugate Chemistry. 24:1345-1355 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc400157z |
| Popis: | The construction of new nanotools is presented here using the example of fluorescent semiconductor nanocrystals, quantum dots (QDs). In this study, the implementation of the new lipid oligonucleotide conjugate-functionalized quantum dots (LON-QDs) is realized in four steps: (i) the synthesis of the lipid oligonucleotide conjugates (LONs), (ii) the encapsulation of QDs by nucleolipids and LONs, (iii) the study of the duplex formation of LON-QDs with the complementary ON partners, and (iv) the cellular uptake of the LON-QD platform and hybridization with the target ONs (microRNA and miR-21). |
| Databáze: | OpenAIRE |
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