Filamentous Fungi Producing l-Asparaginase with Low Glutaminase Activity Isolated from Brazilian Savanna Soil
| Autor: | Marcela Medeiros de Freitas, Danilo Batista Pinho, Adalberto Pessoa, Kellen Cruvinel, Pérola Oliveira Magalhães, Letícia Santos Abrunhosa, João Inácio, Edivaldo Ximenes Ferreira Filho, Paula Monteiro Souza, Samuel Leite Cardoso |
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| Rok vydání: | 2021 |
| Předmět: |
Penicillium sizovae
l-asparaginase Microorganism Fusarium fujikuroi species complex Pharmaceutical Science Fusarium proliferatum acute lymphoblastic leukemia Glutaminase activity Article Pharmacy and materia medica hemic and lymphatic diseases Food science Essential amino acid chemistry.chemical_classification biology Plackett–Burman design filamentous fungi Penicillium food and beverages BIOTECNOLOGIA biology.organism_classification RS1-441 Enzyme chemistry |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Pharmaceutics Volume 13 Issue 8 Pharmaceutics, Vol 13, Iss 1268, p 1268 (2021) |
| ISSN: | 1999-4923 |
| Popis: | l-asparaginase is an enzyme used as treatment for acute lymphoblastic leukemia (ALL) due to its ability to hydrolyze l-asparagine, an essential amino acid synthesized by normal cells unlike neoplastic cells. The adverse effects of l-asparaginase formulations are associated with its glutaminase activity and bacterial origin therefore, it is important to find new sources of l-asparaginase-producing eukaryotic microorganisms with low glutaminase activity. This work evaluated the biotechnological potential of filamentous fungi isolated from Brazilian Savanna soil and plants for l-asparaginase production. Thirty-nine isolates were screened for enzyme production using the plate assay, followed by measuring enzymatic activity in cells after submerged fermentation. The variables influencing l-asparaginase production were evaluated using Plackett–Burman design. Cell disruption methods were evaluated for l-asparaginase release. Penicillium sizovae 2DSST1 and Fusarium proliferatum DCFS10 showed the highest l-asparaginase activity levels and the lowest glutaminase activity levels. Penicillium sizovae l-asparaginase was repressed by carbon sources, whereas higher carbon concentrations enhanced l-asparaginase by F. proliferatum. Maximum enzyme productivity, specific enzyme yield and the biomass conversion factor in the enzyme increased after Plackett–Burman design. Freeze-grinding released 5-fold more l-asparaginase from cells than sonication. This study shows two species, which have not yet been reported, as sources of l-asparaginase with possible reduced immunogenicity for ALL therapy. |
| Databáze: | OpenAIRE |
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