Fluorescent in situ hybridization studies in multiple myeloma
| Autor: | Ozge Ozalp Yuregir, Zerrin Yilmaz, Feride Iffet Sahin, Hakan Ozdogu, E. Kizilkilic, Sema Karakus |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Bone Marrow Cells In situ hybridization Biology Molecular cytogenetics Chromosome regions medicine Humans Multiple myeloma In Situ Hybridization Fluorescence Aged Chromosome Aberrations Chromosomes Human Pair 14 medicine.diagnostic_test Chromosomes Human Pair 13 Chromosome Karyotype Hematology Middle Aged medicine.disease Molecular biology medicine.anatomical_structure Cytogenetic Analysis Female Bone marrow Multiple Myeloma Fluorescence in situ hybridization Chromosomes Human Pair 17 |
| Zdroj: | Hematology (Amsterdam, Netherlands). 14(2) |
| ISSN: | 1607-8454 |
| Popis: | Conventional cytogenetic analysis and fluorescence in situ hybridization (FISH) results of bone marrow samples of 36 multiple myeloma (MM) patients at the time of diagnosis have been evaluated. Three probes for chromosome 13q (RB1, D13S319, D13S25), one for 14q32 (IgH) and one for 17p13 (p53) have been used for hybridization with fixed cells. Twenty patients (55.5%) had normal karyotypes, whereas eight (22.2%) had numerical or structural chromosomal abnormalities. We did not find metaphases for chromosome analysis in eight (22.2%) patients. Fluorescence in situ hybridization analyses revealed at least one or more abnormal results in 25 (69.5%) cases, whereas 11(30.5%) cases had no abnormal findings. 14q32 rearrangement was the most common finding in FISH analyses and has been detected in 21 cases (58.3%). 13q deletion and 17p deletion have been detected in 11 (30.5%) and 5 (13.9%) cases, respectively. Fluorescence in situ hybridization studies including 14q32 and 17p13 chromosome regions may yield quite significant results during clinical follow-up of MM. |
| Databáze: | OpenAIRE |
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