Phylogenetic analysis of human coronavirus NL63 circulating in Italy
| Autor: | Maria Rosaria Capobianchi, E. Schifano, M.S. Zaniratti, Giuseppina Cappiello, Marina Selleri, Claudia Minosse, Alberto Spanò, Giuseppe Gerna, Francesco Nicola Lauria, Vincenzo Puro, Giulia Campanini, Gina Gualano |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Genes Viral EA endotracheal aspirates CoV coronavirus medicine.disease_cause Lower respiratory tract infection Nidovirales Phylogeny Coronavirus hMPV human metapneumovirus Aged 80 and over Phylogenetic analysis biology Phylogenetic tree Reverse Transcriptase Polymerase Chain Reaction virus diseases SP sputum FLU influenzavirus HCoV-NL63 CAP community-acquired pneumonia respiratory system Middle Aged Infectious Diseases Italy Female BAL bronchoalveolar lavage Coronavirus Infections BP bronchopneumonia Human coronavirus NL63 Adult S spike hCoV human coronavirus Adolescent ARF acute respiratory failure RF respiratory failure Virus Article Viral Proteins Human metapneumovirus stomatognathic system Phylogenetics Virology medicine Humans SARS severe acute respiratory syndrome Aged AdV adenovirus LRT lower respiratory tract Human coronaviruses Sequence Analysis DNA biology.organism_classification medicine.disease COPD chronic obstructive pulmonary disease RSV respiratory syncytial virus HRV Human rhinoviruses PIV parainfluenzavirus |
| Zdroj: | Journal of Clinical Virology |
| ISSN: | 1873-5967 1386-6532 |
| Popis: | Background Five known human coronaviruses infect the human respiratory tract: HCoV-OC43, HCoV-229E, SARS-CoV, HCoV-NL63 and HCoV-HKU1. Objectives To evaluate the prevalence of HCoV-NL63 in hospitalized adult patients and to perform molecular characterization of Italian strains. Study Design HCoV-NL63 was sought by RT-PCR in 510 consecutive lower respiratory tract (LRT) samples, collected from 433 Central-Southern Italy patients over a 1-year period. Phylogenetic analysis was performed by partial sequencing of S and ORF1a. Additional S sequences from Northern Italy were included in the phylogenetic trees. Results HCoV-NL63 was detected in 10 patients (2.0%) with symptomatic respiratory diseases, mainly during winter. Phylogenetic analysis indicated a certain degree of heterogeneity in Italian isolates. The ORF1a gene clustering in phylogenetic trees did not match with that of the S gene. Conclusions As observed by others, HCoV-NL63 is often associated with another virus. Phylogenetic characterization of HCoV-NL63 circulating in Italy indicates that this virus circulates as a mixture of variant strains, as observed in other countries. |
| Databáze: | OpenAIRE |
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