Polymerase Chain Reaction Detection of Leishmania kDNA from the Urine of Peruvian Patients with Cutaneous and Mucocutaneous Leishmaniasis
| Autor: | Diego A. Espinosa, Ana Pilar Ramos, Jorge Arevalo, Flor Calderon, Braulio M. Valencia, Donald E. Low, Andrea K. Boggild, Nicolas Veland, Alejandro Llanos-Cuentas |
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| Rok vydání: | 2011 |
| Předmět: |
Leishmaniasis
Mucocutaneous Male Pathology protozoal DNA polymerase chain reaction urinalysis Urine diagnostic kit law.invention cysteine proteinase Diagnostic specimens law Peru Healthy volunteers genetics heat shock protein 70 Leishmania guyanensis Child restriction fragment length polymorphism Polymerase chain reaction Aged 80 and over Leishmania skin leishmaniasis predictive value protozoal protein mannose phosphate isomerase DNA Kinetoplast skin examination smear Articles Middle Aged urine unclassified drug kinetoplast DNA Infectious Diseases Child Preschool Female purl.org/pe-repo/ocde/ford#3.03.06 [https] Adult medicine.medical_specialty Adolescent Mucocutaneous zone skin test Leishmaniasis Cutaneous Biology Leishmania braziliensis non parasitology Young Adult Cutaneous leishmaniasis Virology parasitic diseases medicine Humans controlled study diagnostic test accuracy study parasite identification urine culture Leishmania peruviana Aged aspiration isolation and purification Mucocutaneous leishmaniasis school child medicine.disease biology.organism_classification major clinical study sensitivity and specificity cysteine proteinase b Parasitology |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 84:556-561 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.2011.10-0556 |
| Popis: | We hypothesized that Leishmania kDNA may be present in urine of patients with cutaneous leishmaniasis (CL). Urine samples and standard diagnostic specimens were collected from patients with skin lesions. kDNA polymerase chain reaction (PCR) was performed on samples from patients and 10 healthy volunteers from non-endemic areas. Eighty-six of 108 patients were diagnosed with CL and 18 (21%) had detectable Leishmania Viannia kDNA in the urine. Sensitivity and specificity were 20.9% (95% confidence interval [CI] 12.3-29.5%) and 100%. Six of 8 patients with mucocutaneous involvement had detectable kDNA in urine versus 12 of 78 patients with isolated cutaneous disease (P < 0.001). L. (V.) braziliensis (N = 3), L. (V.) guyanensis (N = 6), and L. (V.) peruviana (N = 3) were identified from urine. No healthy volunteer or patient with an alternate diagnosis had detectable kDNA in urine. Sensitivity of urine PCR is sub-optimal for diagnosis. On the basis of these preliminary data in a small number of patients, detectable kDNA in urine may identify less localized forms of infection and inform treatment decisions. |
| Databáze: | OpenAIRE |
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