Oxidative Stress Protection and Glutathione Metabolism in Response to Hydrogen Peroxide and Menadione in Riboflavinogenic Fungus Ashbya gossypii

Autor: S. Kavitha, T.S. Chandra
Rok vydání: 2014
Předmět:
GPX1
Time Factors
GPX3
Riboflavin
Glutathione reductase
Bioengineering
Biology
Response Elements
GPX4
Eremothecium
Applied Microbiology and Biotechnology
Biochemistry
Antioxidants
Bioinformatics
Fungus protection
Gene expression
Genes
Hydrogen peroxide
Metabolism
Nucleic acids
Oxidation
Oxygen
Peroxides
Physiology
Polymerase chain reaction
RNA
Transcription
CAT
LPX
MRNA level
SOD
STRE/YRE
Peptides
catalase
copper zinc superoxide dismutase
CTT1 protein
glutathione
glutathione disulfide
glutathione peroxidase
glutathione reductase
glutathione transferase
GSH1 protein
hydrogen peroxide
lipid peroxide
membrane lipid
membrane lipid peroxide
menadione
messenger RNA
peroxide
protein
RIB3 protein
riboflavin
superoxide
superoxide dismutase
transcription factor
transcription factor MSN2
transcription factor Yap1
unclassified drug
antioxidant
Article
Ashbya gossypii
cell protection
cellular stress response
concentration (parameters)
controlled study
DNA responsive element
enzyme activity
enzyme induction
fungal biomass
fungus growth
gene expression
glutathione metabolism
lipid peroxidation
nonhuman
oxidative stress
reverse transcription polymerase chain reaction
sequence alignment
stress
biomass
biosynthesis
cytology
dose response
drug effects
extracellular space
gene expression regulation
genetics
metabolism
time
Biomass
Dose-Response Relationship
Drug
Extracellular Space
Gene Expression Regulation
Glutathione
Hydrogen Peroxide
Oxidative Stress
Transcription Factors
Vitamin K 3
chemistry.chemical_compound
Molecular Biology
chemistry.chemical_classification
Lipid peroxide
Glutathione peroxidase
General Medicine
chemistry
Glutathione disulfide
Biotechnology
Zdroj: Applied Biochemistry and Biotechnology. 174:2307-2325
ISSN: 1559-0291
0273-2289
Popis: Ashbya gossypii is a plant pathogen and a natural overproducer of riboflavin and is used for industrial riboflavin production. A few literature reports depict a link between riboflavin overproduction and stress in this fungus. However, the stress protection mechanisms and glutathione metabolism are not much explored in A. gossypii. In the present study, an increase in the activity of catalase and superoxide dismutase was observed in response to hydrogen peroxide and menadione. The lipid peroxide and membrane lipid peroxide levels were increased by H2O2 and menadione, indicating oxidative damage. The glutathione metabolism was altered with a significant increase in oxidized glutathione (GSSG), glutathione peroxidase (GPX), glutathione S transferase (GST), and glutathione reductase (GR) and a decrease in reduced glutathione (GSH) levels in the presence of H2O2 and menadione. Expression of the genes involved in stress mechanism was analyzed in response to the stressors by semiquantitative RT-PCR. The messenger RNA (mRNA) levels of CTT1, SOD1, GSH1, YAP1, and RIB3 were increased by H2O2 and menadione, indicating the effect of stress at the transcriptional level. A preliminary bioinformatics study for the presence of stress response elements (STRE)/Yap response elements (YRE) depicted that the glutathione metabolic genes, stress genes, and the RIB genes hosted either STRE/YRE, which may enable induction of these genes during stress. � 2014, Springer Science+Business Media New York.
Databáze: OpenAIRE
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