Rationalisation of a mechanism for sensing single point variants in target DNA using anthracene-tagged base discriminating probes
| Autor: | Dario M. Bassani, Gediminas Jonusauskas, John Wilkie, Jean-Louis H. A. Duprey, Christian Ludwig, Neil Spencer, Joseph S. Vyle, Alison Rodger, James H. R. Tucker, Eva I. Hyde, Zheng-yun Zhao |
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| Přispěvatelé: | University of Birmingham [Birmingham], Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ondes et Matière d'Aquitaine (LOMA), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Macquarie University, Queen's University [Belfast] (QUB) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Computational biology
Molecular Dynamics Simulation 010402 general chemistry Polymorphism Single Nucleotide 01 natural sciences Biochemistry Nucleobase Molecular dynamics chemistry.chemical_compound Physical and Theoretical Chemistry Anthracenes Anthracene Base Sequence Staining and Labeling 010405 organic chemistry Oligonucleotide Organic Chemistry DNA 0104 chemical sciences chemistry Duplex (building) Molecular Probes Nucleic Acid Conformation [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] Single point Linker |
| Zdroj: | Organic and Biomolecular Chemistry Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2018, 16 (35), pp.6576-6585. ⟨10.1039/c8ob01710g⟩ Duprey, J-L H A, Bassani, D M, Hyde, E I, Jonusauskas, G, Ludwig, C, Rodger, A, Spencer, N, Vyle, J S, Wilkie, J, Zhao, Z-Y & Tucker, J H R 2018, ' Rationalisation of a mechanism for sensing single point variants in target DNA using anthracene-tagged base discriminating probes ', Organic & biomolecular chemistry, vol. 16, no. 35, pp. 6576-6585 . https://doi.org/10.1039/C8OB01710G, https://doi.org/10.1039/c8ob01710g |
| ISSN: | 1477-0520 1477-0539 |
| Popis: | International audience; The labelling of DNA oligonucleotides with signalling groups that give a unique response to duplex formation depending on the target sequence is a highly effective strategy in the design of DNA-based hybridisation sensors. A key challenge in the design of these so-called base discriminating probes (BDPs) is to understand how the local environment of the signalling group affects the sensing response. The work herein describes a comprehensive study involving a variety of photophysical techniques, NMR studies and molecular dynamics simulations, on anthracene-tagged oligonucleotide probes that can sense single base changes (point variants) in target DNA strands. A detailed analysis of the fluorescence sensing mechanism is provided, with a particular focus on rationalising the high dependence of this process on not only the linker stereochemistry but also the site of nucleobase variation within the target strand. The work highlights the various factors and techniques used to respectively underpin and rational-ise the BDP approach to point variant sensing, which relies on different responses to duplex formation rather than different duplex binding strengths. |
| Databáze: | OpenAIRE |
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