Age-related changes in the number of cresyl-violet-stained, parvalbumin and NMDAR 2B expressing neurons in the human spiral ganglion
| Autor: | Tara Sankar Roy, Charanjeet Kaur, Shubhi Saini, D.N. Bhardwaj, Indra Pal, Punit Kumar, Hem Chandra Sati, Tony George Jacob |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Aging Adolescent Presbycusis Stereology Receptors N-Methyl-D-Aspartate 03 medical and health sciences Cresyl violet chemistry.chemical_compound Young Adult 0302 clinical medicine Internal medicine otorhinolaryngologic diseases medicine Cadaver Humans Child Cochlea Spiral ganglion Aged Neurons biology Staining and Labeling Glutamate receptor Age Factors Infant Newborn Infant Middle Aged medicine.disease Sensory Systems Benzoxazines 030104 developmental biology Endocrinology medicine.anatomical_structure Parvalbumins nervous system chemistry Child Preschool biology.protein NMDA receptor Female sense organs Spiral Ganglion 030217 neurology & neurosurgery Parvalbumin |
| Zdroj: | Hearing research. 388 |
| ISSN: | 1878-5891 |
| Popis: | Animal-studies associate age-related hearing loss (presbycusis) with decreasing number of spiral ganglion neurons (SGNs) in Rosenthal’s canal (RC) of cochlea. The excitatory neurotransmitter for SGNs is glutamate (through its receptor NMDAR 2B), which can be neurotoxic through Ca2+ overload. Neurotoxicity is balanced by calcium-binding proteins (CBPs) like Parvalbumin (PV), which is the predominant CBP of the SGNs. To estimate the volume of the RC and total number of SGNs that are immunoreactive to PV and NMDAR 2B, we used unbiased stereology in 35 human cochleae derived from cadavers of persons from 2nd to 8th decade of life (subsequently statistically divided into two groups) and compared them to the total number of cresyl violet (CV) stained SGNs. We also estimated the volume of individual neurons and their nuclei. Regression analysis was made on estimated parameters against age. Hierarchical-cluster analysis was done on the neuronal against neuronal nuclear volumes.The average volume of the RC did not change with increasing age (p = 0.4115). The total number of SGNs (CV-stained and those separately expressing PV and NMDAR 2B) significantly decreased with age (p |
| Databáze: | OpenAIRE |
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