CircRNA circ_0067934 promotes tumor growth and metastasis in hepatocellular carcinoma through regulation of miR-1324/FZD5/Wnt/β-catenin axis
| Autor: | Fenfang Gui, Zihua Luo, Yong Ni, Qian Zhu, Jinghua Wu, Guiyu Lu, Dongwei Zhang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Biophysics Mice Nude Apoptosis Biochemistry Metastasis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Cell Line Tumor medicine Animals Humans Neoplasm Invasiveness Wnt Signaling Pathway Molecular Biology Mice Inbred BALB C Gene knockdown Chemistry Liver Neoplasms Wnt signaling pathway Cell Biology medicine.disease Frizzled Receptors digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Liver Cell culture 030220 oncology & carcinogenesis Catenin Disease Progression Cancer research Signal transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 497:626-632 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2018.02.119 |
| Popis: | Recently, increasing evidences demonstrate that circular RNAs (circRNAs) exert very important functions in the progression of human cancers. However, the functions and molecular mechanism of circ_0067934 in hepatocellular carcinoma (HCC) are largely unknown. In the present study, we found that the expression of circ_0067934 was significantly upregulated in HCC tissues and cell lines compared to adjacent normal tissues. Furthermore, we showed that circ_0067934 knockdown remarkably suppressed the proliferation, migration and invasion of Hep3B and HuH7 cells while inducing their apoptosis. In terms of mechanism, we found that circ_0067934 directly suppressed miR-1324, which targeted the 3′-UTR of FZD5 mRNA and subsequently downregulated the Wnt/β-catenin signaling pathway in HCC. Through rescue experiments, we demonstrated that circ_0067934 enhanced the proliferation, migration and invasion of HCC cells by the inhibition of miR-1324 and concomitant activation of FZD5/Wnt/β-catenin signaling pathway. In summary, the circ_0067934/miR-1324/FZD5/β-catenin signaling axis might serve as a promising therapeutic target for HCC intervention. |
| Databáze: | OpenAIRE |
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