A useful methodology to select lipase-catalyzed transesterification aiming biodiesel application
| Autor: | Marita Gimenez Pereira, Ana Claudia Vici, Andressa Tironi Vieira, Antonio Carlos Ferreira Batista, Taila Angélica da Silva, Fernanda Dell Antonio Facchini, M.F. de Oliveira, Maria de Lourdes Teixeira de Moraes Polizeli, João Atílio Jorge |
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| Rok vydání: | 2016 |
| Předmět: |
Biodiesel
Materials science Waste management biology lcsh:Biotechnology FUNGOS Transesterification Pulp and paper industry lcsh:TD1-1066 Catalysis Biodiesel production lcsh:TP248.13-248.65 Carbon source biology.protein Response surface methodology Lipase lcsh:Environmental technology. Sanitary engineering lcsh:Science (General) Corn oil lcsh:Q1-390 |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Revista Brasileira de Engenharia de Biossistemas, Vol 10, Iss 1, Pp 01-13 (2016) |
| Popis: | The application of lipases in various fields has been notably increased in the last few decades and qualitative/quantitative improvements need to be done. However, many methodologies of screening are described in order to find a good lipase producer and statistical optimization is a necessary tool to improve lipase production. In this work, an isolation of filamentous fungi lipase producers and a transesterification capacity screening was evaluated. Four fungi were chosen to the transesterification reaction assays and the best fungus selected was submitted to a submerged fermentation. Parameters of the culture medium were optimized using response surface methodology. Selected liquid medium was SR at 30 °C, 72 h, 100 rpm. Corn oil was the best carbon source and together with Tween 80 increased two-fold the lipase activity. After the experimental design, the new medium optimized were 3.5-fold higher than the original liquid medium and was composed by 0.5% corn oil, 0.012% MgSO4.7H2O, 0.015% KH2PO4, 0.05% NH4H2PO4. Hence, the lipase produced proved its transesterification capacity and can be used for biodiesel production. |
| Databáze: | OpenAIRE |
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