Plasticity of the RNA kink turn structural motif
| Autor: | Sarah V. Lipchock, Jesse C. Cochrane, Alexandra H. Antonioli, Scott A. Strobel |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Genetics Base Sequence Molecular Sequence Data RNA Conformation Bent molecular geometry Intron Azoarcus RNA Biology Plasticity Crystallography X-Ray biology.organism_classification Article Introns RNA Bacterial Mutation Biophysics Nucleic Acid Conformation Structural motif Molecular Biology Ribonucleoprotein |
| Zdroj: | RNA. 16:762-768 |
| ISSN: | 1469-9001 1355-8382 |
| DOI: | 10.1261/rna.1883810 |
| Popis: | The kink turn (K-turn) is an RNA structural motif found in many biologically significant RNAs. While most examples of the K-turn have a similar fold, the crystal structure of the Azoarcus group I intron revealed a novel RNA conformation, a reverse kink turn bent in the direction opposite that of a consensus K-turn. The reverse K-turn is bent toward the major grooves rather than the minor grooves of the flanking helices, yet the sequence differs from the K-turn consensus by only a single nucleotide. Here we demonstrate that the reverse bend direction is not solely defined by internal sequence elements, but is instead affected by structural elements external to the K-turn. It bends toward the major groove under the direction of a tetraloop–tetraloop receptor. The ability of one sequence to form two distinct structures demonstrates the inherent plasticity of the K-turn sequence. Such plasticity suggests that the K-turn is not a primary element in RNA folding, but instead is shaped by other structural elements within the RNA or ribonucleoprotein assembly. |
| Databáze: | OpenAIRE |
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