Optimization of cryopreservation protocols for cooled-transported stallion semen
| Autor: | B. N. Lister, David J. Hurley, Igor F. Canisso, M.S. Ferrer, Roberto A. Palomares, K. Kline, Robyn E. Ellerbrock, Giorgia Podico |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
endocrine system Semen urologic and male genital diseases Cryopreservation law.invention Specimen Handling 03 medical and health sciences Semen quality fluids and secretions 0302 clinical medicine Endocrinology Animal science Cryoprotective Agents Food Animals law Freezing Animals Horses Acrosome 030219 obstetrics & reproductive medicine urogenital system Chemistry Extender 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Sperm Membrane integrity Animal Science and Zoology Semen Preservation |
| Zdroj: | Animal reproduction science. 221 |
| ISSN: | 1873-2232 |
| Popis: | Freezing cooled-transported semen allows veterinarians and breeders to collect and process the semen of stallions on farm, and then ship the semen to a semen freezing center. There, however, is a lack of standardization of shipping and freezing protocols. The objectives were to optimize and simplify protocols to freeze cooled-shipped semen. In Experiment 1, cooled-transported semen was centrifuged at room temperature or 5 °C before freezing. Sperm variables (motility, membrane integrity, acrosome integrity, membrane fluidity) were evaluated before and after freezing. Centrifugation temperature had no effect on post-thaw semen quality. In Experiment 2, cooled-transported semen was centrifuged at room temperature and cryopreserved in three semen freezing extenders. With use of the improved modified French formula, there was less post-thaw total and progressive motility compared with use of Botucrio or the improved lactose-EDTA formula (P0.0001). Semen cryopreserved in the improved modified French formula also had a lesser percentage of sperm with intact membranes compared with lactose-EDTA, and a greater percentage of sperm with capacitation-like changes compared with Botucrio (P0.0001). In Experiment 3, semen diluted in each extender was frozen conventionally or placed directly in a -80 °C ultra-freezer. Freezing in the ultra-freezer resulted in a lesser post-thaw sperm motility, but not membrane and acrosome integrity and capacitation-like changes. In conclusion, centrifugation and addition of freezing extender to cooled transported semen can be performed at room temperature or 5 °C. The Botucrio and lactose-EDTA formula are recommended for conventional cryopreservation of cooled-transported stallion semen as compared with the modified French formula. |
| Databáze: | OpenAIRE |
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