High mobilization of CD133+/CD34+ cells expressing HIF-1α and SDF-1α in septic abdominal surgical patients

Autor: Nicoletta Mangialetto, Daniela La Bella, Gaetano Palumbo, Flavia Marchese, Olga Cela, Antonella Cotoia, Sabrina Altamura, Nazzareno Capitanio, Gilda Cinnella, Vincenzo Lizzi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
medicine.medical_specialty
Stromal cell
CD34
Gastroenterology
Sepsis
lcsh:RD78.3-87.3
03 medical and health sciences
0302 clinical medicine
Cell Movement
Internal medicine
Abdomen
medicine
Humans
030212 general & internal medicine
Progenitor cell
Endothelial progenitor cells
Hypoxia inducible factor-1α
Aged
Aged
80 and over
Mobilization
business.industry
Septic shock
030208 emergency & critical care medicine
Middle Aged
Hypoxia-Inducible Factor 1
alpha Subunit
medicine.disease
Chemokine CXCL12
Stromal cell-derived factor-1α
Anesthesiology and Pain Medicine
medicine.anatomical_structure
Hypoxia-inducible factors
lcsh:Anesthesiology
Postoperative abdominal laparoscopic patients
embryonic structures
cardiovascular system
Female
Laparoscopy
Bone marrow
business
Hematopoietic stem cells
Research Article
Zdroj: BMC Anesthesiology, Vol 20, Iss 1, Pp 1-8 (2020)
BMC Anesthesiology
ISSN: 1471-2253
Popis: Background The control of endothelial progenitor cells (CD133+/CD34+ EPCs) migrating from bone marrow to peripheral blood is not completely understood. Emerging evidence suggests that stromal cell-derived factor-1α (SDF-1α) mediates egression of EPCs from bone marrow, while the hypoxia inducible factor (HIF) transcriptional system regulates SDF-1α expression. Our study aimed to investigate the time course of circulating CD133+/CD34+ EPCs and its correlation with the expression of HIF-1α protein and SDF-1α in postoperative laparoscopic abdominal septic patients. Methods Postoperative patients were divided in control (C group) and septic group (S group) operated immediately after the diagnosis of sepsis/septic shock. Blood samples were collected at baseline (0), 1, 3 and 7 postoperative days for CD133+/CD34+ EPCs count expressing or not the HIF-1α and SDF-1α analysis. Results Thirty-two patients in S group and 39 in C group were analyzed. In C group CD133+/CD34+ EPCs count remained stable throughout the study period, increasing on day 7 (173 [0–421] /μl vs baseline: P = 0.04; vs day 1: P = 0.002). In S group CD133+/CD34+ EPCs count levels were higher on day 3 (vs day 1: P = 0.006 and day 7: P = 0.026). HIF-1α expressing CD133+/CD34+ EPCs count decreased on day 1 as compared with the other days in C group (day 0 vs 1: P = 0.003, days 3 and 7 vs 1: P = 0.008), while it was 321 [0–1418] /μl on day 3 (vs day 1; P = 0.004), and 400 [0–587] /μl on day 7 in S group. SDF-1α levels were higher not only on baseline but also on postoperative day 1 in S vs C group (219 [124–337] pg/ml vs 35 [27–325] pg/ml, respectively; P = 0.01). Conclusion Our results indicate that sepsis in abdominal laparoscopic patients might constitute an additional trigger of the EPCs mobilization as compared with non-septic surgical patients. A larger mobilization of CD133+/CD34+ EPCs, preceded by enhanced plasmatic SDF-1α, occurs in septic surgical patients regardless of HIF-1α expression therein. Trial registration ClinicalTrials.gov no. NCT02589535. Registered 28 October 2015.
Databáze: OpenAIRE
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