Meniscal tissue engineering via 3D printed PLA monolith with carbohydrate based self-healing interpenetrating network hydrogel
| Autor: | Akriti Sharma, Rama Shanker Verma, J. Vasantha Kumar, Santosh Gupta, Piyush Kumar Gupta, Vineeta Sharma |
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| Rok vydání: | 2020 |
| Předmět: |
Scaffold
3d printed Materials science Biocompatibility Alginates macromolecular substances 02 engineering and technology Biochemistry 03 medical and health sciences Structural Biology Animals Humans Meniscus Rats Wistar Monolith Molecular Biology 030304 developmental biology 0303 health sciences geography geography.geographical_feature_category Tissue Engineering Tissue Scaffolds Meniscal tissue technology industry and agriculture Mesenchymal Stem Cells General Medicine 021001 nanoscience & nanotechnology Rats Transplantation Self-healing Printing Three-Dimensional Self-healing hydrogels 0210 nano-technology Chondrogenesis Biomedical engineering |
| Zdroj: | International Journal of Biological Macromolecules. 162:1358-1371 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.07.238 |
| Popis: | Failure of bioengineered meniscus implant after transplantation is a major concern owing to mechanical failure, lack of chondrogenic capability and patient specific design. In this article, we have, for the first time, fabricated a 3D printed scaffold with carbohydrate based self-healing interpenetrating network (IPN) hydrogels-based monolith construct for load bearing meniscus tissue. 3D printed PLA scaffold was surface functionalized and embedded with self-healing IPN hydrogel for interfacial bonding further characterized by micro CT. Using collagen (C), alginate (A) and oxidized alginate (ADA), we developed self-healing IPN hydrogels with dual crosslinking (Ca2+ based ionic crosslinking and Schiff base (A-A, A-ADA)) capability and studied their physicochemical properties. Further, we studied human stem cells behaviour and chondrogenic differentiation potential within these IPN hydrogels. In-vivo heterotopic implantation confirmed biocompatibility of the monolith showing the feasibility of using carbohydrate based IPN hydrogel embedded in 3D printed scaffold for meniscal tissue development. |
| Databáze: | OpenAIRE |
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