The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes
| Autor: | Anthony E. G. Cass, Chee-Seng Toh, Evelyne Simon, Philip N. Bartlett, Catherine M. Halliwell |
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| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
L-Lactate Dehydrogenase Immobilized enzyme Bioelectric Energy Sources Cell Mutant Biophysics Dehydrogenase Peptide General Medicine Enzymes Immobilized chemistry.chemical_compound medicine.anatomical_structure Aniline Enzyme chemistry Biochemistry Mutagenesis Electrochemistry medicine NAD+ kinase Physical and Theoretical Chemistry Peptides Electrodes |
| Zdroj: | Bioelectrochemistry. 55:21-23 |
| ISSN: | 1567-5394 |
| Popis: | The immobilization of the mutants of L-lactate dehydrogenase (LDH) on poly(aniline) (PANi) composite films has been investigated. Mutants possessing peptide tags of varying charge and nucleophilicity were created to probe the nature of the interaction between the protein and PANi. These results are significant for the development of a 'generic' approach to the immobilization of enzymes and other proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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