Genome characterization of a flexuous rod-shaped mycovirus, Botrytis virus X, reveals high amino acid identity to genes from plant ‘potex-like’ viruses
| Autor: | Robyn L. J. Howitt, Richard L. S. Forster, Ross E. Beever, Michael N. Pearson |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Complementary food.ingredient viruses Molecular Sequence Data RNA-dependent RNA polymerase Genome Viral Biology Virus Plant Viruses Open Reading Frames food Virology RNA Viruses Amino Acid Sequence ORFS Gene Peptide sequence Phylogeny Allexivirus Botrytis cinerea Genetics Sequence Homology Amino Acid food and beverages General Medicine Plants biology.organism_classification Potexvirus DNA Viral Mycovirus Botrytis |
| Zdroj: | Archives of Virology. 151:563-579 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | This study reports the molecular characterization of a flexuous rod-shaped mycovirus, Botrytis virus X (BVX), infecting the plant-pathogenic fungus, Botrytis cinerea. BVX contains a ssRNA genome of 6966 nucleotides, and a poly(A) tract at or very near the 3' terminus. Computer analysis of the genomic cDNA sequence of BVX revealed five potential open reading frames (ORFs). ORF1 showed significant amino acid sequence identity to the replicase proteins of plant 'potex-like' viruses, including 73% identity to the RNA-dependent RNA polymerase (RdRp) region of the allexivirus, garlic virus A (GarV-A). The C-terminal region of ORF3 shared amino acid homology with plant 'potex-like' coat proteins. The remaining ORFs did not reveal significant homology with known protein sequences. BVX differs substantially from Botrytis virus F (BVF), another flexuous rod-shaped mycovirus characterized from the same B. Cinerea isolate. It is proposed that the mycovirus BVX belongs to a new, as yet unassigned genus in the plant 'potex-like' virus group, distinct from BVF. |
| Databáze: | OpenAIRE |
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