Upregulation of TNF-alpha production by IFN-gamma and LPS in cultured canine keratinocytes: application to monosaccharides effects
Autor: | J. Viac, P. Bourdeau, Hugues Gatto, C. Ibisch, C. Cadiot |
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Rok vydání: | 2006 |
Předmět: |
Keratinocytes
Lipopolysaccharides Time Factors Lipopolysaccharide Cell Antineoplastic Agents Biology chemistry.chemical_compound Interferon-gamma Mice Dogs Cell Line Tumor medicine Animals Humans Viability assay Autocrine signalling Cells Cultured General Veterinary Tumor Necrosis Factor-alpha Monosaccharides Keratinocyte activation General Medicine Recombinant Interferon Gamma Molecular biology Recombinant Proteins Up-Regulation medicine.anatomical_structure chemistry Gene Expression Regulation Cell culture Tumor necrosis factor alpha Female |
Zdroj: | Veterinary research communications. 31(7) |
ISSN: | 0165-7380 |
Popis: | Activated keratinocytes play a key role in the cutaneous immune system by their interactions with other cell types through the production of cytokines with both autocrine and paracrine activity. But there is little knowledge about epidermal cytokines in the dog. In this study, cultured canine keratinocytes were stimulated by human recombinant interferon gamma (IFN-gamma) and lipopolysaccharide (LPS) and cell supernatants were tested for tumour necrosis factor alpha (TNF-alpha) concentration using a cell viability assay on a murine cell line. We show that IFN-gamma in combination with LPS significantly increases TNF-alpha secretion by canine keratinocytes. The best stimulations were obtained using confluent cultures and the association of IFN-gamma (400 ng/ml) and LPS (40 microg/ml). The experimental protocol we describe represents a new method for studying keratinocyte activation and its modulation in the dog. We provide an example of application of our method: the study of the effects of different monosaccharides on canine keratinocyte activation. |
Databáze: | OpenAIRE |
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