Somal Size of Immunolabeled Pyramidal Cells in the Prefrontal Cortex of Subjects with Schizophrenia
| Autor: | Allan R. Sampson, Jaime G. Maldonado-Avilés, David A. Lewis, Qiang Wu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Psychosis Neurofilament Central nervous system Fluorescent Antibody Technique Prefrontal Cortex Neuronal nuclear Biology Immunoenzyme Techniques Reference Values medicine Humans Prefrontal cortex Biological Psychiatry Aged Cell Size Analysis of Variance Immunoperoxidase Techniques Pyramidal Cells food and beverages Middle Aged medicine.disease Control subjects medicine.anatomical_structure nervous system Schizophrenia Neuroscience |
| Zdroj: | Biological Psychiatry. 60:226-234 |
| ISSN: | 0006-3223 |
| DOI: | 10.1016/j.biopsych.2005.10.028 |
| Popis: | Background Although the somal volume of Nissl-stained deep layer 3 pyramidal cells is reduced in prefrontal cortex area 9 of subjects with schizophrenia, the subset of large pyramidal cells immunoreactive (IR) for nonphosphorylated neurofilament protein (NNFP) is not. Consequently, we hypothesized that the somal volume of another subset of pyramidal cells immunoreactive for neuronal calcium binding protein-1 (Necab-1) is significantly reduced in schizophrenia. Methods We labeled Necab-1-IR pyramidal neurons using immunoperoxidase techniques and estimated the mean somal volume in deep layer 3 of area 9 in 13 matched pairs of control and schizophrenic subjects. Identical studies were conducted for pyramidal neurons immunoreactive for neuronal nuclear protein (Neu-N), which is present in all neurons. Results In subjects with schizophrenia, neither the mean somal volume of Necab-1-IR pyramidal neurons nor of Neu-N-IR pyramidal neurons was significantly different from control subjects. In addition, the mean somal volume of Neu-N-IR cells was larger than that of Nissl-stained cells in both subject groups, and the magnitude of this difference was greater for the subjects with schizophrenia. Conclusions These findings suggest that immunoperoxidase techniques are associated with an overestimation of the volume of labeled neurons. This confound appears to interact with disease state, and thus obscures differences between diagnostic groups. |
| Databáze: | OpenAIRE |
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