Calcium overload induces C6 rat glioma cell apoptosis in sonodynamic therapy

Autor:	Qi Zhan, Fubin Ren, Jing-Ye Liu, Zhi-Qiang Chen, Zheng Huang, Wu Yue, Song-Bin Fu, Jianhua Li
Rok vydání:	2011
Předmět:	Necrosis Ultrasonic Therapy Immunoblotting chemistry.chemical_element Apoptosis Calcium Sarcoplasmic Reticulum Calcium-Transporting ATPases medicine Tumor Cells Cultured Animals Radiology Nuclear Medicine and imaging Fluorometry Egtazic Acid Calcium metabolism chemistry.chemical_classification Reactive oxygen species Photosensitizing Agents Radiological and Ultrasound Technology biology Caspase 3 Cytochrome c Sonodynamic therapy Cytochromes c Glioma Intracellular Membranes Molecular biology Acetylcysteine Rats Hematoporphyrins Biochemistry chemistry biology.protein medicine.symptom Reactive Oxygen Species Intracellular
Zdroj:	International journal of radiation biology. 87(10)
ISSN:	1362-3095
Popis:	Our aim was to study calcium overload-induced apoptosis and its relation to reactive oxygen species (ROS) in rat C6 glioma cells after sonodynamic treatment (SDT).Hematoporphyrin monomethyl ether (HMME) was used as the sonosensitizer. The concentration of intracellular Ca(2+) ([Ca(2+)](i)) was measured by fluorometry. Apoptosis and necrosis rates were evaluated by a flow cytometry. Moreover, sarcoplasmic reticulum Ca(2+) -ATPase (SERCA(2)), cytochrome c (cyto-c) and cleaved caspase-3 were investigated by immunoblotting.Our study indicated that [Ca(2 +)](i) and ROS increased in cells of SDT group, the apoptosis rate, quantity of cyto-c and cleaved caspase-3 markedly increased after SDT. Furthermore, N-Acetyl-L-cysteine (NAC) or 1,2-bisethane-N,N,N',N'-tetraacetic acid tetrakis ester (BAPTA-AM) could decrease the apoptosis rate, the release of cyto-c and cleaved caspase-3 in SDT group, SERCA(2) degradation was found in SDT group and could also be prevented by the addition of NAC.Our results show that HMME-SDT can induce C6 cell death through both necrosis and apoptosis. ROS in C6 cells play a decisive role in HMME-SDT-induced cell death. The endoplasmic reticulum (ER) may be a major target of HMME-SDT, ROS can induce SERCA(2) degradation, causing the elevation of [Ca(2+)](i).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dde2db3d4c7ae588271814887c5a09de https://pubmed.ncbi.nlm.nih.gov/21961969 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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