Comparison of cytotoxicity and genotoxicity effects of silver nanoparticles on human cervix and breast cancer cell lines
| Autor: | N Girón-Vazquez, Karla Juarez-Moreno, Josué D. Mota-Morales, L L Perez-Mozqueda, E B Gonzalez, Maritza Roxana Garcia-Garcia, Rocio Alejandra Chavez-Santoscoy, Nina Bogdanchikova, Alexey Pestryakov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Silver Cell Survival DNA damage Health Toxicology and Mutagenesis Metal Nanoparticles Uterine Cervical Neoplasms Apoptosis Breast Neoplasms 02 engineering and technology Toxicology medicine.disease_cause HeLa 03 medical and health sciences Cell Line Tumor medicine Humans Cytotoxic T cell Cytotoxicity Dose-Response Relationship Drug biology Chemistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology Comet assay 030104 developmental biology Cell culture Nanotoxicology Female Comet Assay Reactive Oxygen Species 0210 nano-technology Genotoxicity DNA Damage |
| Zdroj: | Europe PubMed Central ResearcherID |
| ISSN: | 1477-0903 0960-3271 |
| DOI: | 10.1177/0960327116675206 |
| Popis: | The wide application of silver nanoparticles (AgNPs) has pointed out the need to evaluate their potential risk and toxic effects on human health. Herein, the cytotoxic effects of Argovit™ AgNPs were evaluated on eight cancer cell lines. Further cytotoxic studies were performed in gynecological cancer cell lines from cervical (HeLa) and breast (MDA-MB-231 and MCF7) cancer. In both cases, the half maximal inhibitory concentration (IC50) of AgNPs produced the formation of reactive oxygen species (ROS) after 24 h of incubation, but it was not statistically significant compared with untreated cells. However, HeLa, MDA-MB-231, and MCF7 cells treated with the maximal IC of AgNPs induced the formation of ROS either at 12 or 24 h of incubation. Genotoxicity achieved by comet assay in HeLa, MDA-MB-231, and MCF7 cells revealed that exposure to IC50 of AgNPs does not induced noticeable DNA damage in the cells. However, the IC of AgNPs provoked severe DNA damage after 12 and 24 h of exposure. We conclude that, Argovit (polyvinylpyrrolidone-coated AgNPs) induce a cytotoxic effect in a time and dose-dependent manner in all the eight cancer cell lines tested. Nevertheless, the genotoxic effect is mainly restricted by the concentration effect. The results contribute to explore new therapeutic applications of AgNPs for malignances in murine models and to study in deep the cytotoxic and genotoxic effects of AgNPs in healthy cells at the surrounding tissue of the neoplasia. |
| Databáze: | OpenAIRE |
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