Differential Responses of Antioxidative System during the Interaction of Soursop Fruits (
| Autor: | Guillermo Berumen-Varela, José Orlando Jiménez-Zurita, Verónica Alhelí Ochoa-Jiménez, Alejandro Rubio-Melgarejo, Paloma Patricia Casas-Junco, Pedro Ulises Bautista-Rosales, Rosendo Balois-Morales |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant DPPH medicine.medical_treatment antioxidant activity Plant Science 01 natural sciences Polyphenol oxidase Article Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound medicine Food science polyphenol oxidase Annona muricata Ecology Evolution Behavior and Systematics ABTS Ecology biology Chemistry Botany food and beverages biology.organism_classification superoxide dismutase early response total soluble phenols 030104 developmental biology QK1-989 biology.protein Postharvest gene expression Fusarium solani 010606 plant biology & botany |
| Zdroj: | Plants Volume 10 Issue 7 Plants, Vol 10, Iss 1432, p 1432 (2021) |
| ISSN: | 2223-7747 |
| Popis: | Soursop fruit (Annona muricata L.) production is diminished by the attack of pathogens such as Nectria haematococca. However, the fruit–pathogen interaction at the biochemical and molecular levels is still unknown. The objective of this study was to analyze the response of the soursop fruit to the presence of N. haematococca during postharvest storage. Soursop fruits were inoculated with the pathogen and total phenolic compounds, antioxidant capacity by Ferric reducing/antioxidant power (FRAP), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+), and 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH•), as well as enzymatic activity and transcript levels of polyphenol oxidase (PPO) and superoxide dismutase (SOD), were evaluated at 1, 3, and 5 days of storage. The noninoculated fruits were the controls of the experiment. The highest total phenol content was recorded on day one in the inoculated fruits. FRAP, ABTS, and DPPH activity presented the highest values on day three in the control fruits. Inoculated fruits recorded the highest PPO activity on day five and a five-fold induction in the PPO transcript on day three. SOD activity showed a decrease during the days of storage and 10-fold induction of SOD transcript on day three in the inoculated fruits. Principal component analysis showed that total phenols were the variable that contributed the most to the observed variations. Furthermore, a positive correlation between total phenols and SOD activity, PPO expression, and SOD expression, as well as between DPPH and FRAP, was recorded. The results showed a differential response in antioxidant capacity, enzymatic activity, and gene expression during the interaction of soursop fruits–N. haematococca at postharvest storage. |
| Databáze: | OpenAIRE |
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