The Non-Lantibiotic Bacteriocin Garvicin Q Targets Man-PTS in a Broad Spectrum of Sensitive Bacterial Genera
| Autor: | Paulina Wirtek, Dzung B. Diep, Tamara Aleksandrzak-Piekarczyk, Aleksandra Tymoszewska |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lactococcus Science 030106 microbiology Sequence Homology Mannose Article Subclass Microbiology 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Bacteriocins Bacteriocin Amino Acid Sequence Phosphoenolpyruvate Sugar Phosphotransferase System Multidisciplinary biology Lactococcus lactis food and beverages PEP group translocation Lantibiotics biology.organism_classification Anti-Bacterial Agents 030104 developmental biology chemistry Biochemistry Lactococcus garvieae bacteria Medicine |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
| Popis: | Mannose phosphotransferase system (Man-PTS) is the main mannose permease in bacteria but it is also a known receptor for subclass IIa bacteriocins (pediocin-like group) as well as subclass IId lactococcin A (LcnA) and lactococcin B (LcnB) (LcnA-like group). Subclass IIa bacteriocins exhibit a strong activity against Listeria spp. but they are not against Lactococcus spp. In contrast, the LcnA-like bacteriocins act only against Lactococcus lactis strains. Garvicin Q (GarQ) is a subclass IId bacteriocin with minor similarity to LcnA-like bacteriocins and a relatively broad antimicrobial spectrum including, among others, Listeria and Lactococcus spp. To identify the GarQ receptor, we obtained GarQ-resistant mutants of Lactococcus garvieae IBB3403 and L. lactis IL1403 and sequenced their genomes that revealed mutations in genes encoding the membrane-bound Man-PTS IIC or IID subunits encoded by ptnCD in L. lactis and manCD in L. garvieae. This is the first time that a bacteriocin outside the pediocin- and LcnA-like groups is shown to target Man-PTS. The interaction between GarQ and Man-PTS may occur through a new binding pattern involving specific amino acids highly conserved among the GarQ-sensitive bacterial species located in the N-terminal part and extracellular loops of subunit IID and in transmembrane region of IIC. |
| Databáze: | OpenAIRE |
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