Far less frequent mutations inras genes than in thep53 gene in skin tumors of xeroderma pigmentosum patients

T transition at a dipyrimidine (5'-CT) sequence and could have been produced by solar ultraviolet light. The mutated ras gene did not have the ability to transform NIH/3T3 cells. In three tumors, multiple base substitutions were detected in exon 1 of the Ki-ras and N-ras genes. These results and our previous work on p53 gene mutations suggest that mutations in ras genes are far less frequent than in the p53 gene in the skin tumors in XP patients and that ras genes are less important in skin tumorigenesis in XP patients than is the p53 gene. -->

ISSN:	1098-2744 0899-1987
DOI:	10.1002/mc.2940110207
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dddf7b6d61ae06a1fc1184c976308e45 https://doi.org/10.1002/mc.2940110207
Rights:	CLOSED
Přírůstkové číslo:	edsair.doi.dedup.....dddf7b6d61ae06a1fc1184c976308e45
Autor:	Hiraku Takebe, Mohamed Zghal, Takashi Yagi, Mayumi Sato, Yanjun Lu, Chikako Nishigori
Rok vydání:	1994
Předmět:	Adult Cancer Research Skin Neoplasms Xeroderma pigmentosum Adolescent Molecular Sequence Data Biology Gene mutation medicine.disease_cause Mice Exon Ultraviolet light medicine Animals Humans Child Molecular Biology Gene Polymorphism Single-Stranded Conformational Xeroderma Pigmentosum Mutation Base Sequence integumentary system Point mutation 3T3 Cells Genes p53 medicine.disease Molecular biology Genes ras Carcinoma Basal Cell Carcinoma Squamous Cell Cancer research Carcinogenesis
Zdroj:	Molecular Carcinogenesis. 11:98-105
ISSN:	1098-2744 0899-1987
DOI:	10.1002/mc.2940110207
Popis:	Mutations in Ha-ras, Ki-ras, and N-ras genes in squamous and basal cell carcinomas in patients with xeroderma pigmentosum (XP) were examined by the polymerase chain reaction followed by single-strand conformation polymorphism analysis and direct base sequencing. No mutation was detected in codons 12, 13, and 61 of the ras genes in XP skin tumors. This was in contrast with previous findings of a high frequency of mutation in the p53 gene in skin tumors in XP patients. A novel mutation in codon 6 of the Ki-ras gene was detected in a squamous cell carcinoma. The mutation was a C-->T transition at a dipyrimidine (5'-CT) sequence and could have been produced by solar ultraviolet light. The mutated ras gene did not have the ability to transform NIH/3T3 cells. In three tumors, multiple base substitutions were detected in exon 1 of the Ki-ras and N-ras genes. These results and our previous work on p53 gene mutations suggest that mutations in ras genes are far less frequent than in the p53 gene in the skin tumors in XP patients and that ras genes are less important in skin tumorigenesis in XP patients than is the p53 gene.
Databáze:	OpenAIRE
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