Plasmodium falciparum: Nitric Oxide Modulates Heme Speciation in Isolated Food Vacuoles
| Autor: | Nicolas Collin, Graciela R. Ostera, Clarissa Teixeira, José M. C. Ribeiro, Juliana Sa, Fuyuki Tokumasu, Gudrun S. Lukat-Rodgers, Sanjai Kumar, Jennifer Hume, Kenton R. Rodgers |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Erythrocytes
Immunology Immunoblotting Plasmodium falciparum Vacuole Heme Biology Nitric Oxide Spectrum Analysis Raman Article chemistry.chemical_compound Mice Cytochrome b5 Food vacuole Animals Humans Crystallization Erythrocytes/parasitology Heme/chemistry Heme/metabolism Immune Sera/diagnostic use Microscopy Fluorescence Nitric Oxide/metabolism Plasmodium falciparum/genetics Plasmodium falciparum/metabolism Solubility Vacuoles/chemistry Vacuoles/metabolism Cytochrome b5 reductase Reactive nitrogen species Hemozoin Immune Sera General Medicine Cytosol Infectious Diseases Biochemistry chemistry Vacuoles Parasitology |
| Zdroj: | Experimental Parasitology, vol. 127, no. 1, pp. 1-8 |
| Popis: | Nitric oxide (NO) and NO-derived reactive nitrogen species (RNS) are present in the food vacuole (FV) of Plasmodium falciparum trophozoites. The product of PFL1555w, a putative cytochrome b(5), localizes in the FV membrane, similar to what was previously observed for the product of PF13_0353, a putative cytochrome b(5) reductase. These two gene products may contribute to NO generation by denitrification chemistry from nitrate and/or nitrite present in the erythrocyte cytosol. The possible coordination of NO to heme species present in the food vacuole was probed by resonance Raman spectroscopy. The spectroscopic data revealed that in situ generated NO interacts with heme inside the intact FVs to form ferrous heme nitrosyl complexes that influence intra-vacuolar heme solubility. The formation of heme nitrosyl complexes within the FV is a previously unrecognized factor that could affect the equilibrium between soluble and crystallized heme within the FV in vivo. |
| Databáze: | OpenAIRE |
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