Cytokinin N-glucosides: Occurrence, Metabolism and Biological Activities in Plants
| Autor: | Roberta Filepová, H. Tucker Hallmark, Aaron M. Rashotte, Břetislav Brzobohatý, Eva Pokorná, Lukáš Spíchal, Lenka Zahajská, Tomáš Hluska, Pavel Mazura, Ondřej Novák, Tomáš Filipi, Václav Motyka, Ondřej Plíhal, Petr Galuszka, Petre I. Dobrev, Jiří Malbeck, Lenka Záveská Drábková, Katarína Holubová |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine senescence lcsh:QR1-502 Arabidopsis 01 natural sciences Biochemistry lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound cytokinin β-D-glucosidase Zm-p60.1 oat Molecular Biology Gene [2-3H]tZ9G Zea mays Oxidase test biology trans-zeatin fungi Metabolism biology.organism_classification Metabolic pathway 030104 developmental biology chemistry Cytokinin cis-zeatin Signal transduction N-glucosides cytokinin oxidase/dehydrogenase Function (biology) 010606 plant biology & botany |
| Zdroj: | Biomolecules, Vol 11, Iss 24, p 24 (2021) Biomolecules Volume 11 Issue 1 |
| Popis: | Cytokinins (CKs) are a class of phytohormones affecting many aspects of plant growth and development. In the complex process of CK homeostasis in plants, N-glucosylation represents one of the essential metabolic pathways. Its products, CK N7- and N9-glucosides, have been largely overlooked in the past as irreversible and inactive CK products lacking any relevant physiological impact. In this work, we report a widespread distribution of CK N-glucosides across the plant kingdom proceeding from evolutionary older to younger plants with different proportions between N7- and N9-glucosides in the total CK pool. We show dramatic changes in their profiles as well as in expression levels of the UGT76C1 and UGT76C2 genes during Arabidopsis ontogenesis. We also demonstrate specific physiological effects of CK N-glucosides in CK bioassays including their antisenescent activities, inhibitory effects on root development, and activation of the CK signaling pathway visualized by the CK-responsive YFP reporter line, TCSv2::3XVENUS. Last but not least, we present the considerable impact of CK N7- and N9-glucosides on the expression of CK-related genes in maize and their stimulatory effects on CK oxidase/dehydrogenase activity in oats. Our findings revise the apparent irreversibility and inactivity of CK N7- and N9-glucosides and indicate their involvement in CK evolution while suggesting their unique function(s) in plants. |
| Databáze: | OpenAIRE |
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