The effects of nickel(II) complexes with imidazole derivatives on pyocyanin and pyoverdine production by Pseudomonas aeruginosa strains isolated from cystic fibrosis
| Autor: | Marcin Drabik, Sławomir Wąsik, Katarzyna Gałczyńska, Karol Szary, Michał Arabski, Krystyna Kurdziel, Wioletta Adamus-Białek, Aneta Wegierek-Ciuk, Anna Lankoff |
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| Rok vydání: | 2015 |
| Předmět: |
Cystic Fibrosis
Pyocyanine Biology Microscopy Atomic Force medicine.disease_cause Cystic fibrosis General Biochemistry Genetics and Molecular Biology Cell Line Microbiology chemistry.chemical_compound Pyocyanin Nickel medicine Humans A549 cell Pyoverdine Pseudomonas aeruginosa Imidazoles Biofilm medicine.disease Spectrometry Fluorescence chemistry Biochemistry Cell culture Oligopeptides |
| Zdroj: | ResearcherID |
| ISSN: | 1734-154X 0001-527X |
| DOI: | 10.18388/abp.2015_1124 |
| Popis: | Pseudomonas aeruginosa infection is problematic in patients with cystic fibrosis (CF). P. aeruginosa secretes a diversity of pigments, such as pyocyanin and pyoverdine. The aim of this study was to evaluate the effects of complexes of nickel(II) ([Ni(iaa)2(H2O)2]·H2O (iaa = imidazole-4-acetate anion), [Ni(1-allim)6](NO3)2 (1-allim = 1-allylimidazole) and NiCl2 on pyocyanin and pyoverdine production by 23 strains of P. aeruginosa isolated from cystic fibrosis under growth conditions specific for the CF respiratory system. The antibacterial effects and biophysical properties of the tested substances were measured by spectrofluorometric techniques, as well as by laser interferometry, confocal and atomic force microscopy. The cytotoxic properties of all compounds were measured by Annexin/IP assay against A549 cells. All tested compounds have no effect on pyocyanin production and decrease the pyoverdine secretion in about 40% of tested P. aeruginosa strains at non-cytotoxic range of concentrations. Imidazole-4-acetate anion and 1-allylimidazole have good diffusion properties in the mature P. aeruginosa PAO1 biofilm. In conclusion, the tested nickel(II) complexes do not have clinical implications in P. aeruginosa eradication in cystic fibrosis. The diffusion properties of 1-allylimidazole and imidazole-4-acetate and their lack of effect on A549 cells suggest that they might be considered for chemical synthesis with other transition metals. |
| Databáze: | OpenAIRE |
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