The mouse Clc1/myotonia gene: ETn insertion, a variable AATC repeat, and PCR diagnosis of alleles
| Autor: | Niels Wedemeyer, Olga Antropova, Monika Gronemeier, Volker Schnülle, Jörg W. Bartsch, Harald Jockusch |
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| Rok vydání: | 1997 |
| Předmět: |
Transposable element
DNA Mutational Analysis Molecular Sequence Data Muscle Proteins Sequence Homology Biology Polymerase Chain Reaction Myotonia Exon Mice Chloride Channels Genetics Coding region Animals Humans Amino Acid Sequence Allele Gene Alleles Genomic organization Repetitive Sequences Nucleic Acid Polymorphism Genetic Base Sequence Intron Chromosome Mapping DNA Exons Molecular biology Null allele Introns Rats Genes DNA Transposable Elements |
| Zdroj: | Mammalian genome : official journal of the International Mammalian Genome Society. 8(10) |
| ISSN: | 0938-8990 |
| Popis: | Myotonias are muscle diseases in which the function of the muscular chloride channel ClC-1 is impaired. Null alleles of the corresponding Clc1 gene on mouse chromosome (Chr) 6 provide animal models for human myotonias. It was shown that the allele adr (Clc1 adr ) is due to an insertion of an ETn type transposon that is transcribed and leads to multiple splicing events; the allele mto (Clc1 adr-mto ) involves a stop codon near the N-terminus. We have determined the genomic organization of the mouse Clc1 gene and the sequence requirements for the transposon insertion in the Clc1 adr allele. The mouse Clc1 gene is composed of 23 exons, ranging from 39 to 372 bp, and spans approximately 23 kb of genomic DNA. The exon/intron organization is highly homologous to that of the human CLCN1 gene; the homology of the coding sequence is 97% to rat and 89% to human. In the adr allele the ETn transposon is inserted into intron 12, the largest intron. Whereas the 5′ and 3′ LTR sequences of the ETn transposon are homologous to those reported for other insertional mutations of the mouse, no consensus motif for an insertion target site could be defined. On the basis of flanking sequences, we provide duplex PCR diagnoses for the adr, adr-mto, and wild-type alleles of Clc1. Close to the 3′ end of intron 12, a tetranucleotide repeat (AATC)n was found that is polymorphic between mouse species Mus musculus, M. molossinus, M. castaneus, and M. spretus, and can thus be used for chromosomal mapping studies. |
| Databáze: | OpenAIRE |
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