Use of the Capripoxvirus homologue of Vaccinia virus 30kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: Development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus

Autor: Antony George Luckins, Hafsa Madani, David B. Wallace, Velý Gulyaz, Roland Silber, Charles Euloge Lamien, Eeva S.M. Tuppurainen, Emmanuel Albina, Christian Le Goff, Tajelser Adam, Adama Diallo, Mehdi El Harrak, Philippe Caufour
Přispěvatelé: FAO Sub-regional Office for Eastern Africa [Addis Ababa, Ethiopie] (FAO), Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO), Contrôle des maladies animales exotiques et émergentes (UMR CMAEE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Austrian Agency for Health and Food Safety, Agricultural Research Council (ARC), University of Pretoria [South Africa], Pendik Vet Control & Res Inst, Partenaires INRAE, AFRC Institute of Animal Physiology and Genetics Research, Institut National de la Médecine Vétérinaire [Mohammadia, Algérie] (INMV), Minist Sci & Technol, Lytix Biopharma, MAE [LABOVET 2003-24]
Rok vydání: 2011
Předmět:
Identification
Maladie de la peau
Virologie
[SDV]Life Sciences [q-bio]
Mouton
Poxviridae Infections
L73 - Maladies des animaux
medicine.disease_cause
Polymerase Chain Reaction
Capripoxvirus
0403 veterinary science
chemistry.chemical_compound
RNA polymerase subunit
Immunologie
Lumpy skin disease
Orthopoxvirus
Phylogeny
Chèvre
Sequence Deletion
Genetics
0303 health sciences
biology
Goats
DNA-Directed RNA Polymerases
04 agricultural and veterinary sciences
General Medicine
Lumpy skin disease virus
3. Good health
méthode
Génotype
Genotyping
Genotype
040301 veterinary sciences
Molecular Sequence Data
Cattle Diseases
Sheep Diseases
Vaccinia virus
Sheep pox
Goat pox
Microbiology
Host Specificity
Virus
03 medical and health sciences
Species Specificity
medicine
Animals
L50 - Physiologie et biochimie animales
Poxviridae
Diagnostic
Amino Acid Sequence
030304 developmental biology
Test biologique
Sheeppox
Goat Diseases
Sheep
General Veterinary
Goatpox virus
biology.organism_classification
Virology
chemistry
DNA
Viral
Cattle
U30 - Méthodes de recherche
Vaccinia
Sequence Alignment
Virose
Zdroj: Veterinary Microbiology
Veterinary Microbiology, Elsevier, 2011, 149 (1-2), pp.30-39. ⟨10.1016/j.vetmic.2010.09.038⟩
ISSN: 0378-1135
Popis: International audience; Sheep poxvirus (SPPV), Goat poxvirus (GTPV) and Lumpy skin disease virus (LSDV) are Capripoxviruses (CaPVs) responsible for causing severe poxvirus disease in sheep, goats and cattle, respectively. Serological differentiation of CaPVs is not possible and strain identification has relied on the implicitly accepted hypothesis that the viruses show well defined host specificity. However, it is now known that cross infections can occur and authentication of identity based on the host animal species from which the strain was first isolated, is not valid and should be replaced with molecular techniques to allow unequivocal strain differentiation. To identify a diagnostic target for strain genotyping, the CaPV homologue of the Vaccinia virus E4L gene which encodes the 30 kDa DNA-dependant RNA polymerase subunit, RPO30 was analyzed. Forty-six isolates from different hosts and geographical origins were included. Most CaPVs fit into one of the three different groups according to their host origins: the SPPV, the GTPV and the LSDV group. A unique 21-nucleotide deletion was found in all SPPV isolates which was exploited to develop a RPO30-based classical PCR test to differentiate SPPV from GTPV that will allow rapid differential diagnosis of disease during CaPV outbreaks in small ruminants.
Databáze: OpenAIRE
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