Periostin alters transcriptional profile in a rat model of isoproterenol-induced cardiotoxicity
| Autor: | Tuba Devrim, Alparslan Kadir Devrim, Yonca Betil Kabak, Mahmut Sözmen |
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| Přispěvatelé: | Kırıkkale Üniversitesi, Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Health Toxicology and Mutagenesis Periostin Toxicology medicine.disease_cause Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Gene expression medicine Animals rat periostin 030102 biochemistry & molecular biology Chemistry isoproterenol General Medicine Cell cycle Cardiotoxicity Cell biology Disease Models Animal 030220 oncology & carcinogenesis Gene chip analysis gene expression Tumor necrosis factor alpha Signal transduction Transcriptome Cell Adhesion Molecules microarray Oxidative stress Extracellular matrix organization |
| Popis: | Sozmen, Mahmut/0000-0001-7976-4051 WOS: 000456381600010 PubMed: 30303030 Periostin is an extracellular matrix protein from the fasciclin family that guides cellular trafficking and extracellular matrix organization. Periostin stimulates mature cardiomyocytes to reenter the cell cycle. The molecular mechanism behind such stimulation remains to be explored. A DNA microarray technology constituting 30,429 gene-level probe sets was utilized to investigate effects of recombinant murine periostin peptide on the gene expression pattern in a rat model of isoproterenol (ISO)-induced myocardial injury. The experiment was performed on 84 adult male Sprague-Dawley rats in four groups (n = 21): (1) control group, (2) only periostin applied group, (3) ISO cardiotoxicity group, and (4) ISO + periostin group. The experiment was continued for 28 days, and rats were killed on days 1, 7, and 28 (n = 7). Microarray analyses revealed that periostin significantly altered the expression of at least +/- 2-fold of 2474 genes in the ISO + periostin group compared to the ISO cardiotoxicity group of which 521 genes altered out of 30,429 gene-level probe sets. Ingenuity pathway analysis indicated that multiple pathway networks were affected by periostin, with predominant changes occurring in the expression of genes involved in oxidative phosphorylation, oxidative stress, fatty acid metabolism, and TNF-alpha NF-kappa B signaling pathways. These findings indicate that periostin alters gene expression profile in the ISO-induced myocardial injury and modulates local myocardial inflammation, possibly mitigating inflammation through TNF-alpha NF-kappa B signaling pathway along with a decreased Casp7 activity and apoptotic cell death. Ondokuz Mayis University Scientific Research and Development Support Program, Samsun [PYO.VET.1901.13]; Turkish Scientific Research Council (TUBITAK-TOVAG), Ankara, Turkey [114O734] The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This project was financially supported by Ondokuz Mayis University Scientific Research and Development Support Program (Project No.: PYO.VET.1901.13), Samsun, and Turkish Scientific Research Council (TUBITAK-TOVAG; Project No.: 114O734), Ankara, Turkey. |
| Databáze: | OpenAIRE |
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