Investigating the Diversity and Host Range of Novel Parvoviruses from North American Ducks Using Epidemiology, Phylogenetics, Genome Structure, and Codon Usage Analysis
| Autor: | Joost T. P. Verhoeven, Sheena Roul, Gregory J. Robertson, Hannah J. Munro, Suzanne C. Dufour, Andrew S. Lang, Davor Ojkic, Marta Canuti, Hugh Whitney |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Subfamily viruses 030106 microbiology lcsh:QR1-502 Animals Wild chaphamaparvovirus Genome Viral insect viruses Viral Nonstructural Proteins lcsh:Microbiology Host Specificity Parvoviridae Article densovirus Parvoviridae Infections 03 medical and health sciences dinucleotide frequencies Phylogenetics Virology Animals Clade avian viruses Phylogeny virus discovery biology Phylogenetic tree codon usage Parvovirus Ambidensovirus parvovirus virus diseases biology.organism_classification 030104 developmental biology Infectious Diseases Ducks Evolutionary biology Codon usage bias DNA Viral Seasons Densovirus |
| Zdroj: | Viruses Viruses, Vol 13, Iss 193, p 193 (2021) Volume 13 Issue 2 |
| ISSN: | 1999-4915 |
| Popis: | Parvoviruses are small single-stranded DNA viruses that can infect both vertebrates and invertebrates. We report here the full characterization of novel viruses we identified in ducks, including two viral species within the subfamily Hamaparvovirinae (duck-associated chapparvovirus, DAC) and a novel species within the subfamily Densovirinae (duck-associated ambidensovirus, DAAD). Overall, 5.7% and 21.1% of the 123 screened ducks (American black ducks, mallards, northern pintail) were positive for DAC and DAAD, respectively, and both viruses were more frequently detected in autumn than in winter. Genome organization and predicted transcription profiles of DAC and DAAD were similar to viruses of the genera Chaphamaparvovirus and Protoambidensovirus, respectively. Their association to these genera was also demonstrated by subfamily-wide phylogenetic and distance analyses of non-structural protein NS1 sequences. While DACs were included in a highly supported clade of avian viruses, no definitive conclusions could be drawn about the host type of DAAD because it was phylogenetically close to viruses found in vertebrates and invertebrates and analyses of codon usage bias and nucleotide frequencies of viruses within the family Parvoviridae showed no clear host-based viral segregation. This study highlights the high parvoviral diversity in the avian reservoir with many avian-associated parvoviruses likely yet to be discovered. |
| Databáze: | OpenAIRE |
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