Harnessing Fluorescent Moenomycin A Antibiotics for Bacterial Cell Wall Imaging Studies
| Autor: | Wei-Chieh Cheng, Chih-Wei Guo, Fan-Chun Meng, Yu-Ling Shih, Pei-Yu Hsieh, Kung-Hsiang Hu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
medicine.drug_class Antibiotics Microbial Sensitivity Tests Peptidoglycan Bacterial growth medicine.disease_cause Biochemistry Bacterial cell structure Cell wall chemistry.chemical_compound Cell Wall medicine Molecular Biology Fluorescent Dyes chemistry.chemical_classification biology Molecular Structure Chemistry Organic Chemistry Optical Imaging biology.organism_classification Anti-Bacterial Agents Enzyme Bambermycins Staphylococcus aureus Molecular Medicine Bacteria |
| Zdroj: | Chembiochem : a European journal of chemical biology. 22(24) |
| ISSN: | 1439-7633 |
| Popis: | The imaging of peptidoglycan (PGN) dynamics in living bacteria facilitates the understanding of PGN biosynthesis and wall-targeting antibiotics. The main tools for imaging bacterial PGN are fluorescent probes, such as the well-known PGN metabolic labeling probes. However, fluorescent small-molecule probes for labeling key PGN-synthesizing enzymes, especially for transglycosylases (TGases), remain to be explored. In this work, the first imaging probe for labeling TGase in bacterial cell wall studies is reported. We synthesized various fluorescent MoeA-based molecules by derivatizing the natural antibiotic moenomycin A (MoeA), and used them to label TGases in living bacteria, monitor bacterial growth and division cycles by time-lapse imaging, and study cell wall growth in the mecA-carrying methicillin-resistant Staphylococcus aureus (MRSA) strains when the β-lactam-based probes were unsuitable. |
| Databáze: | OpenAIRE |
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